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Literature summary for 3.1.4.39 extracted from
- Measurement of lysophospholipase D/autotaxin activity in human serum samples (2007), Clin. Biochem., 40, 274-277.
Application
| Application | Comment | Organism |
|---|---|---|
| diagnostics | lysoPLD assay can be applied to clinical laboratory testing. Serum lysoPLD activity in healthy female subjects is significantly higher than that in male subjects. It seems likely that lysoPLD and its product lysophosphatidic acid participate in female reproductive biology. lysoPLD assay method seems to be promising for laboratory testing because it allows easy handling of the samples | Homo sapiens |
General Stability
| General Stability | Organism |
|---|---|
| lysoPLD activity is found to be very stable, the activity shows no change even after 180-min incubation at 37°C | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| lysophosphatidylcholine + H2O | Homo sapiens | present in the plasma | lysophosphatidic acid + choline | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| blood serum | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
lysoPLD activity is assessed, based on the amount of choline released with lysophosphatidylcholine as the substrate, absorption spectrometry. Amounts of the substrate lysophosphatidylcholine and the product lysophosphatidic acid are also checked simultaneously. Changes in the lysoPLD activity and lysophosphatidylcholine and lysophosphatidic acid concentrations in incubated serum. Concentrations of lysophosphatidylcholine and lysophosphatidic acid increase upon incubation, the levels increase significantly as compared with those in the controls (no incubation) after 60-min incubations. Dramatic increase in the lysophosphatidic acid concentration is observed, and the lysophosphatidic acid level after 180-min incubation is about 15times higher than in the control | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| lysophosphatidylcholine + H2O | present in the plasma | Homo sapiens | lysophosphatidic acid + choline | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| autotaxin | - |
Homo sapiens |
| lysophospholipase D | - |
Homo sapiens |
| lysoPLD | regulates the blood levels of lysophosphatidic acid (LPA) | Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
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