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Literature summary for 3.1.4.38 extracted from
- Properties of a Zn(2+)-glycerophosphocholine cholinephosphodiesterase from bovine brain membranes (1996), Neurochem. Res., 21, 1193-1199.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.016 | - |
p-nitrophenylphosphocholine | - |
Bos taurus |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Bos taurus | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | stimulates to 140% | Bos taurus | |
| Cu2+ | - |
Bos taurus | |
| Mn2+ | is able to stabilize the enzyme | Bos taurus | |
| Zn2+ | stabilizes and stimulates to 96% | Bos taurus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 110000 | - |
gel filtration, SDS-PAGE | Bos taurus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.046 | - |
- |
Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| p-nitrophenylphosphocholine + H2O | - |
Bos taurus | p-nitrophenol + choline phosphate | - |
r | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | 65 | membrane bound enzyme is stable at 45oC | Bos taurus |
| 45 | 65 | 90% loss of activity of the soluble enzyme after 1 h storage at 65oC | Bos taurus |
| 45 | 65 | 62% loss of activity of the membrane bound enzyme after 1 h storage at 65oC | Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 10.5 | - |
- |
Bos taurus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 9 | 11 | membrane bound enzyme is more stable at various pHs | Bos taurus |
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