Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | - |
Streptomyces sanglieri | |
EDTA | 13% activation at 2 mM | Streptomyces sanglieri | |
additional information | poor activation by 2 mM DTT and SDS | Streptomyces sanglieri |
Cloned (Comment) | Organism |
---|---|
gene gpc-cp, DNA and amino acid sequence determination and analysis, sequence comparison and phyogenetic tree, peptide sequencing, molecular cloning in Escherichia coli strain HST08, recombinant expression of the C-terminally His6-tagged enzyme in enzyme-lacking Streptomyces lividans strain 1326 (NBRC15675) | Streptomyces sanglieri |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 80.6% inhibition at 2 mM | Streptomyces sanglieri | |
Cu2+ | complete inhibition at 2 mM | Streptomyces sanglieri | |
Fe2+ | 49.4% inhibition at 2 mM | Streptomyces sanglieri | |
Mn2+ | 20.5% inhibition at 2 mM | Streptomyces sanglieri | |
additional information | no inhibition by 2 mM SDS or DTT, poor inhibition by 2 mm iodoacetamide | Streptomyces sanglieri | |
PMSF | 21.2% inhibition at 2 mM | Streptomyces sanglieri | |
Zn2+ | 99.6% inhibition at 2 mM | Streptomyces sanglieri |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.41 | - |
sn-glycerol-3-phosphocholine | pH 7.2, 37°C | Streptomyces sanglieri |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Streptomyces sanglieri | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 14% activation at 2 mM | Streptomyces sanglieri | |
additional information | the enzyme is metal ion-independent, no effect by 2 mm Mg2+ and K+ | Streptomyces sanglieri | |
Na+ | 11% activation at 2 mM | Streptomyces sanglieri |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
66000 | - |
native enzyme, gel filtration | Streptomyces sanglieri |
70447 | - |
1 * 66000, SDS-PAGE, 1 * 70447, sequence calculation | Streptomyces sanglieri |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sn-glycero-3-phosphocholine + H2O | Streptomyces sanglieri | - |
glycerol + phosphocholine | - |
? | |
sn-glycero-3-phosphocholine + H2O | Streptomyces sanglieri A14 | - |
glycerol + phosphocholine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
no activity in Streptomyces lividans | - |
NBRC15675 | - |
no activity in Streptomyces lividans 1326 | - |
NBRC15675 | - |
Streptomyces sanglieri | U6C6H1 | isolation and identification, gene gpc-cp | - |
Streptomyces sanglieri A14 | U6C6H1 | isolation and identification, gene gpc-cp | - |
Purification (Comment) | Organism |
---|---|
recombinant C-terminally His6-tagged enzyme from Streptomyces lividans strain 1326 by ammonium sulfate fractionation, nickel affinity and hydrophobic interaction chromatography, native extracellular enzyme 53fold from culture supernatant by ammonium sulfate fractionation, dialysis, anion exchange chromatography, and ultrafiltration, followed by hydrophobic interaction chromatography, ultrafiltration, two different steps of anion exxchange chromatography, ultrafiltration, gel filtration, ultrafiltration, cation exchange chromatography, and again ultrafiltration | Streptomyces sanglieri |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
282 | - |
purified recombinant His-tagged enzyme, pH 7.2, 37°C | Streptomyces sanglieri |
583 | - |
purified native enzyme, pH 7.2, 37°C | Streptomyces sanglieri |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-alpha-lysophosphatidylcholine + H2O | - |
Streptomyces sanglieri | ? | - |
? | |
L-alpha-lysophosphatidylcholine + H2O | - |
Streptomyces sanglieri A14 | ? | - |
? | |
additional information | substrate specificity, overview. The enzyme exhibits specificity toward glycerol-3-phosphocholine and glycerol-3-phosphoethanolamine and hydrolyzes glycerol-3-phosphate and lysophosphatidylcholine. The enzyme shows no activity toward any diacylglycerophospholipids and little activity toward other glycerol-3-phosphodiesters and lysophospholipids, no activity with sn-glycerol-3-phosphoserine, L-alpha-lysophosphatidylinositol, L-alpha-lysophosphatidylserine, L-alpha-lysophosphatidylglycerol, 1,2-dipalmitoyl-sn-glycero-3-phosphocholine, 1,2-dimyristoyl-snglycero-3-phosphate, 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-rac-(1-glycerol), L-alpha-phosphatidylinositol, sphingomyelin, beta-acetyl-gamma-sn-hexadecyl-L-alpha-phosphatidylcholine, or 1-O-1'-(Z)-octadecenyl-2-hydroxy-sn-glycero-3-phosphoethanolamine, poor activity with 1-O-1'-(Z)-octadecenyl-2-hydroxy-sn-glycero-3-phosphocholine, L-alpha-lysophosphatidylethanolamine, or 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-L-serine | Streptomyces sanglieri | ? | - |
? | |
additional information | substrate specificity, overview. The enzyme exhibits specificity toward glycerol-3-phosphocholine and glycerol-3-phosphoethanolamine and hydrolyzes glycerol-3-phosphate and lysophosphatidylcholine. The enzyme shows no activity toward any diacylglycerophospholipids and little activity toward other glycerol-3-phosphodiesters and lysophospholipids, no activity with sn-glycerol-3-phosphoserine, L-alpha-lysophosphatidylinositol, L-alpha-lysophosphatidylserine, L-alpha-lysophosphatidylglycerol, 1,2-dipalmitoyl-sn-glycero-3-phosphocholine, 1,2-dimyristoyl-snglycero-3-phosphate, 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-rac-(1-glycerol), L-alpha-phosphatidylinositol, sphingomyelin, beta-acetyl-gamma-sn-hexadecyl-L-alpha-phosphatidylcholine, or 1-O-1'-(Z)-octadecenyl-2-hydroxy-sn-glycero-3-phosphoethanolamine, poor activity with 1-O-1'-(Z)-octadecenyl-2-hydroxy-sn-glycero-3-phosphocholine, L-alpha-lysophosphatidylethanolamine, or 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-L-serine | Streptomyces sanglieri A14 | ? | - |
? | |
sn-glycero-3-phosphocholine + H2O | - |
Streptomyces sanglieri | glycerol + phosphocholine | - |
? | |
sn-glycero-3-phosphocholine + H2O | - |
Streptomyces sanglieri A14 | glycerol + phosphocholine | - |
? | |
sn-glycerol-3-phosphate + H2O | lower activity | Streptomyces sanglieri | glycerol + phosphate | - |
? | |
sn-glycerol-3-phosphate + H2O | lower activity | Streptomyces sanglieri A14 | glycerol + phosphate | - |
? | |
sn-glycerol-3-phosphocholine + H2O | best substrate | Streptomyces sanglieri | glycerol + phosphocholine | - |
? | |
sn-glycerol-3-phosphocholine + H2O | best substrate | Streptomyces sanglieri A14 | glycerol + phosphocholine | - |
? | |
sn-glycerol-3-phosphoethanolamine + H2O | high activity | Streptomyces sanglieri | glycerol + phosphoethanolamine | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 66000, SDS-PAGE, 1 * 70447, sequence calculation | Streptomyces sanglieri |
Synonyms | Comment | Organism |
---|---|---|
GPC-CP | - |
Streptomyces sanglieri |
GPC-CP14 | - |
Streptomyces sanglieri |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Streptomyces sanglieri |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | 60 | activity range with over 95% of maximal activity | Streptomyces sanglieri |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | 45 | completely stable at | Streptomyces sanglieri |
50 | - |
purified extracellular enzyme, 30 min, stable at | Streptomyces sanglieri |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
29 | - |
sn-glycerol-3-phosphocholine | pH 7.2, 37°C | Streptomyces sanglieri |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
- |
Streptomyces sanglieri |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | narrow pH range near pH 7 | Streptomyces sanglieri |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 10.5 | purified extracellular enzyme, 30 min, stable at | Streptomyces sanglieri |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme may be a member of the PLC/acid phosphatase protein superfamily, and the member of a distinct family, unlike glycerophosphocholine phosphodiesterase (EC 3.1.4.2) and alkaline phosphatase (EC 3.1.3.1) | Streptomyces sanglieri |
metabolism | sn-glycerol-3-phosphocholine hydrolysis by the enzme might represent a different metabolic pathway for acquisition of a phosphorus source in actinomycetes | Streptomyces sanglieri |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
20.6 | - |
sn-glycerol-3-phosphocholine | pH 7.2, 37°C | Streptomyces sanglieri |