Cloned (Comment) | Organism |
---|---|
wild-type and mutant CNP2, expression in 293T human kidney fibroblast cells | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
M21L | CNP2 mutant | Rattus norvegicus |
S22A | CNP2 mutant with reduced phosphate incorporation | Rattus norvegicus |
S23A | CNP2 mutant | Rattus norvegicus |
S9A | CNP2 mutant with reduced phosphate incorporation | Rattus norvegicus |
S9A/S22A | CNP2 double mutant without phosphate incorporation | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | abundantly in the cytoplasmic compartments of CNS myelin | Rattus norvegicus | 5737 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46000 | - |
x * 46000, CNP1, x * 48000, CNP2 | Rattus norvegicus |
48000 | - |
x * 46000, CNP1, x * 48000, CNP2 | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Rattus norvegicus | CNP performs an integral role in myelinogenesis, both isoforms likely play complementary roles in the onset of process outgrowth and ultimately myelination of axons | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
Sprague-Dawley rats, 1-2 days old, 2 isoforms: CNP1 and CNP2 differing by a 20-amino acid extension exclusive to CNP2 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | only CNP2 is phosphorylated in vivo, CNP1 not, Ser-9 of CNP2 is phosphorylated by a 4-beta-phorbol 12,13-dibutyrate-sensitive kinase, likely protein kinase C, and Ser-22 appears to be constitutively phosphorylated, phosphorylation of Ser-9 may provide a molecular switching mechanism for modulating the function of CNP2 distinguishing it functionally from CNP1 | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
glial cell | oligodendrocyte-enriched cultures of neonatal rat brain glial cells, CNP2 | Rattus norvegicus | - |
myelin | abundantly in the cytoplasmic compartments of CNS myelin | Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | CNP performs an integral role in myelinogenesis, both isoforms likely play complementary roles in the onset of process outgrowth and ultimately myelination of axons | Rattus norvegicus | ? | - |
? | |
nucleoside 2',3'-cyclic phosphate + H2O | - |
Rattus norvegicus | nucleoside 2'-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 46000, CNP1, x * 48000, CNP2 | Rattus norvegicus |