Cloned (Comment) | Organism |
---|---|
chimaeric PDE5/PDE6 catalytic domain is expressed in Escherichia coli BL21-codon plus cells | Bos taurus |
Crystallization (Comment) | Organism |
---|---|
chimaeric PDE5/PDE6 catalytic domain complexed with sildenafil or 3-isobutyl-1-methylxanthine and the Pgamma-inhibitory peptide Pgamma70-87, hanging drop vapor diffusion method, using 100 mM Tris (pH 8.5), 200 mM MgSO4, 12% (w/v) PEG-3350, and 2.5% (v/v) ethanol, at 4°C | Bos taurus |
Protein Variants | Comment | Organism |
---|---|---|
N661A | the substitution moderately increases the Km value for cGMP hydrolysis but it markedly decreases the potency of PDE5/6cd inhibition by Pgamma inhibitory peptide | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-isobutyl-1-methylxanthine | - |
Bos taurus | |
Pgamma-inhibitory peptide | - |
Bos taurus | |
Pgamma-inhibitory peptide Pgamma63-87 | - |
Bos taurus | |
Pgamma-inhibitory peptide Pgamma70-87 | - |
Bos taurus | |
sildenafil | - |
Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0036 | - |
3',5'-cGMP | chimaeric PDE5/PDE6 catalytic domain, pH and temperature not specified in the publication | Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3',5'-cGMP + H2O | Bos taurus | PDE5 and PDE6 show specificity for cGMP relative to cAMP | 5'-GMP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
NiSO4-charged His-bind resin column chromatography and benzamidine Sepharose column chromatography | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3',5'-cGMP + H2O | PDE5 and PDE6 show specificity for cGMP relative to cAMP | Bos taurus | 5'-GMP | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PDE5 | - |
Bos taurus |
PDE5/PDE6cd | chimaeric PDE5/6 catalytic domain | Bos taurus |
PDE6 | - |
Bos taurus |
phosphodiesterase-5 | - |
Bos taurus |
phosphodiesterase-6 | - |
Bos taurus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.55 | - |
3',5'-cGMP | chimaeric PDE5/PDE6 catalytic domain, pH and temperature not specified in the publication | Bos taurus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000025 | - |
sildenafil | chimaeric PDE5/6 catalytic domain, pH and temperature not specified in the publication | Bos taurus | |
0.0015 | - |
Pgamma-inhibitory peptide | chimaeric PDE5/6 catalytic domain, pH and temperature not specified in the publication | Bos taurus | |
0.0029 | - |
Pgamma-inhibitory peptide Pgamma63-87 | chimaeric PDE5/6 catalytic domain, pH and temperature not specified in the publication | Bos taurus | |
0.0085 | - |
3-isobutyl-1-methylxanthine | chimaeric PDE5/6 catalytic domain, pH and temperature not specified in the publication | Bos taurus | |
0.0121 | - |
Pgamma-inhibitory peptide Pgamma70-87 | chimaeric PDE5/6 catalytic domain, pH and temperature not specified in the publication | Bos taurus |