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Literature summary for 3.1.4.35 extracted from

  • Su, Y.H.; Vacquier, V.D.
    Cyclic GMP-specific phosphodiesterase-5 regulates motility of sea urchin spermatozoa (2006), Mol. Biol. Cell, 17, 114-121.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.4.35

Cloned(Commentary)

Cloned (Comment) Organism
catalytic domain (COOH-terminal half-containing the catalytic domain Thr540-Ile949), expression in bacteria Strongylocentrotus purpuratus

Inhibitors

Inhibitors Comment Organism Structure
3-isobutyl-1-methylxanthine IC50: 0.032 mM Strongylocentrotus purpuratus
dipyridamole IC50: 0.023 mM Strongylocentrotus purpuratus
sildenafil IC50: 0.005 mM Strongylocentrotus purpuratus
T-1032 IC50: 0.041 mM Strongylocentrotus purpuratus
zaprinast IC50: 0.24 mM Strongylocentrotus purpuratus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
 3',5'-cGMP
-
 Strongylocentrotus purpuratus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3',5'-cGMP + H2O Strongylocentrotus purpuratus suPDE5 regulates cGMP levels in sperm, which in turn modulate sperm motility 5'-GMP
-
 ?

Organism

Organism UniProt Comment Textmining
Strongylocentrotus purpuratus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein suPDE5 activity is regulated by phosphorylation. In vitro dephosphorylation of suPDE5 decreases activity by 50% Strongylocentrotus purpuratus

Purification (Commentary)

Purification (Comment) Organism
-
 Strongylocentrotus purpuratus

Source Tissue

Source Tissue Comment Organism Textmining
sperm flagellum
-
 Strongylocentrotus purpuratus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3',5'-cGMP + H2O suPDE5 regulates cGMP levels in sperm, which in turn modulate sperm motility Strongylocentrotus purpuratus 5'-GMP
-
 ?
3',5'-cGMP + H2O specific for 3',5'-cGMP, no activity with 3',5'-cAMP Strongylocentrotus purpuratus 5'-GMP
-
 ?

Synonyms

Synonyms Comment Organism
suPDE5
-
 Strongylocentrotus purpuratus

pH Range

pH Minimum pH Maximum Comment Organism
7 8 3fold increase in activity from pH 7 to pH 8 Strongylocentrotus purpuratus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.005
-
 IC50: 0.005 mM Strongylocentrotus purpuratus sildenafil
0.023
-
 IC50: 0.023 mM Strongylocentrotus purpuratus dipyridamole
0.032
-
 IC50: 0.032 mM Strongylocentrotus purpuratus 3-isobutyl-1-methylxanthine
0.041
-
 IC50: 0.041 mM Strongylocentrotus purpuratus T-1032
0.24
-
 IC50: 0.24 mM Strongylocentrotus purpuratus zaprinast