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Literature summary for 3.1.4.35 extracted from

  • Wang, P.; Wu, P.; Myers, J.G.; Stamford, A.; Egan, R.W.; Billah, M.M.
    Characterization of human, dog and rabbit corpus cavernosum type 5 phosphodiesterases (2001), Life Sci., 68, 1977-1987.
    View publication on PubMed
Search for more literature for 3.1.4.35

Inhibitors

Inhibitors Comment Organism Structure
3-isobutyl-1-methylxanthine 0.0058 mM, 50% inhibition Canis lupus familiaris
3-isobutyl-1-methylxanthine 0.0058 mM, 50% inhibition Homo sapiens
3-isobutyl-1-methylxanthine 0.0054 mM, 50% inhibition Oryctolagus cuniculus
dipyridamole 0.0013 mM, 50% inhibition Canis lupus familiaris
dipyridamole 0.00075 mM, 50% inhibition Homo sapiens
dipyridamole 0.0012 mM, 50% inhibition Oryctolagus cuniculus
sildenafil 0.0000017 mM, 50% inhibition Canis lupus familiaris
sildenafil 0.0000036 mM, 50% inhibition Homo sapiens
sildenafil 0.000003 mM, 50% inhibition Oryctolagus cuniculus
zaprinast 0.0002 mM, 50% inhibition Canis lupus familiaris
zaprinast 0.0003 mM, 50% inhibition Homo sapiens
zaprinast 0.000053 mM, 50% inhibition Oryctolagus cuniculus
Zn2+ complete inhibition above 0.1 mM Canis lupus familiaris
Zn2+ strong inhibition above 0.01 mM, almost complete inhibition at 0.1 mM Homo sapiens
Zn2+ strong inhibition above 0.01 mM, complete inhibition at 0.1 mM Oryctolagus cuniculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0008
-
 cGMP 30°C, pH 7.4 Homo sapiens
0.001
-
 cGMP 30°C, pH 7.4, platelet PDE5 Homo sapiens
0.002
-
 cGMP 30°, pH 7.4 Canis lupus familiaris
0.0023
-
 cGMP 30°C, pH 7.4 Oryctolagus cuniculus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required for activity, approx. 1.5fold activation at 1 mM Oryctolagus cuniculus
Mg2+ required for activity, approx. 2.5fold activation at 1 mM Homo sapiens
Mg2+ required for activity, approx. 2fold activation at 1 mM Canis lupus familiaris
Zn2+ approx. 2.5 fold activation at 0.01 mM, strong inhibition above Homo sapiens
Zn2+ slight activation at 0.01 mM, strong inhibition above Canis lupus familiaris

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
guanosine 3',5'-cyclic phosphate + H2O Homo sapiens
-
 guanosine 5'-phosphate
-
 ?
guanosine 3',5'-cyclic phosphate + H2O Oryctolagus cuniculus
-
 guanosine 5'-phosphate
-
 ?
guanosine 3',5'-cyclic phosphate + H2O Canis lupus familiaris
-
 guanosine 5'-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Canis lupus familiaris
-
-
-
Homo sapiens
-
-
-
Oryctolagus cuniculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Mono Q, partially purified Homo sapiens
Mono Q, partially purified Oryctolagus cuniculus
Mono Q, partially purified Canis lupus familiaris

Source Tissue

Source Tissue Comment Organism Textmining
corpus cavernosum
-
 Homo sapiens
-
corpus cavernosum
-
 Canis lupus familiaris
-
platelet
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
guanosine 3',5'-cyclic phosphate + H2O
-
 Homo sapiens guanosine 5'-phosphate
-
 ?
guanosine 3',5'-cyclic phosphate + H2O
-
 Oryctolagus cuniculus guanosine 5'-phosphate
-
 ?
guanosine 3',5'-cyclic phosphate + H2O
-
 Canis lupus familiaris guanosine 5'-phosphate
-
 ?

Synonyms

Synonyms Comment Organism
PDE5
-
 Homo sapiens
PDE5
-
 Oryctolagus cuniculus
PDE5
-
 Canis lupus familiaris
type 5 phosphodiesterase
-
 Homo sapiens
type 5 phosphodiesterase
-
 Oryctolagus cuniculus
type 5 phosphodiesterase
-
 Canis lupus familiaris

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Homo sapiens
7.5
-
-
 Oryctolagus cuniculus
7.5
-
-
 Canis lupus familiaris

pH Range

pH Minimum pH Maximum Comment Organism
6 8.5 almost no activity at pH 6.0 and 8.5, respectively Homo sapiens
6.5 8.5 almost no activity at pH 6.5 and pH 8.5, respectively Canis lupus familiaris
6.5 8.5 very low activity at pH 6.5 and 8.5, respectively Oryctolagus cuniculus