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Literature summary for 3.1.4.35 extracted from
- Identification and characterization of a new human type 9 cGMP-specific phosphodiesterase splice variant (PDE9A5). Differential tissue distribution and subcellular localization of PDE9A variants (2003), Gene, 314, 15-27.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cloning of cDNA, expression of PDE9A5 in HEK293 cells | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 3-isobutyl-1-methylxanthine | - |
Homo sapiens |  |
| EDTA | 0.5 mM, 35% inhibition of activity in the absence of a divalent metal ion | Homo sapiens | |
| SCH 51866 | 0.0029 mM, 50% inhibition of PDE9A5, 0.0033 mM, 50% inhibition of PDE9A1 | Homo sapiens | |
| sildenafil | 0.011 mM, 50% inhibition of PDE9A5, 0.008 mM, 50% inhibition of PDE9A1 | Homo sapiens | |
| zaprinast | 0.046 mM, 50% inhibition of PDE9A5, 0.043 mM, 50% inhibition of PDE9A1 | Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00025 | - |
cGMP | splice variant PDE9A1 | Homo sapiens | |
| 0.00039 | - |
cGMP | splice variant PDE9A5 | Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | splice variant PDE9A5 | Homo sapiens | 5829 | - |
| nucleus | splice variant PDE9A1 | Homo sapiens | 5634 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | slight activation of PDE9A1 | Homo sapiens | |
| Mg2+ | required for activity, maximal activity at approx. 10 mM | Homo sapiens | |
| Mn2+ | required for activity, maximal activity at approx. 10 mM | Homo sapiens | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| guanosine 3',5'-cyclic phosphate + H2O | Homo sapiens | - |
guanosine 5'-phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | O76083 | splice variants PDE9A5 and PDE9A1 | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Homo sapiens | - |
| colon | - |
Homo sapiens | - |
| heart | - |
Homo sapiens | - |
| kidney | - |
Homo sapiens | - |
| liver | - |
Homo sapiens | - |
| lung | - |
Homo sapiens | - |
| prostate | - |
Homo sapiens | - |
| small intestine | - |
Homo sapiens | - |
| spleen | highest expression levels of splice variants PDE9A5 and PDE9A1, expression levels of PDE9A1 in descending order, spleen, prostate, colon, kidney, brain, small intestine, heart, liver and lung, expression levels of PDE9A5 in descending order, spleen, brain, prostate, colon, small intestine, kidney, lung, heart and liver | Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| guanosine 3',5'-cyclic phosphate + H2O | - |
Homo sapiens | guanosine 5'-phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PDE9A | - |
Homo sapiens |
| type 9 cGMP-specific phosphodiesterase | - |
Homo sapiens |
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