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Literature summary for 3.1.4.3 extracted from

  • Elena, C.; Cerminati, S.; Ravasi, P.; Rasia, R.; Peiru, S.; Menzella, H.; Castelli, M.
    B. cereus phospholipase C engineering for efficient degumming of vegetable oil (2017), Process Biochem., 54, 67-72 .
No PubMed abstract available
Search for more literature for 3.1.4.3

Application

Application Comment Organism
food industry the enzyme is used in vegetable oil refining by enzymatic phospholipid removal (degumming ) Bacillus cereus

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris strain GS115H Bacillus cereus

Protein Variants

Protein Variants Comment Organism
F66W the mutant enzyme can remove up to 90% of phosphatidylethanolamine while retaining its efficiency at hydrolyzing phosphatidylcholine Bacillus cereus
F66Y the mutant enzyme can remove up to 90% of phosphatidylethanolamine while retaining its efficiency at hydrolyzing phosphatidylcholine Bacillus cereus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
14.8
-
 4-nitrophenylphosphorylcholine mutant enzyme F66W, at pH 7.0 and 50°C Bacillus cereus
17.4
-
 4-nitrophenylphosphorylcholine mutant enzyme F66Y, at pH 7.0 and 50°C Bacillus cereus
39.5
-
 4-nitrophenylphosphorylcholine wild type enzyme, at pH 7.0 and 50°C Bacillus cereus

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ contains 3 zinc ions Bacillus cereus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phosphatidylcholine + H2O Bacillus cereus
-
 1,2-diacyl-sn-glycerol + phosphorylcholine
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus cereus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-dihexanoyl-sn-glycero-3-phosphoethanolamine + H2O less efficient substrate Bacillus cereus 1,2-dihexanoyl-sn-glycerol + phosphoethanolamine
-
 ?
4-nitrophenylphosphorylcholine + H2O
-
 Bacillus cereus 4-nitrophenol + phosphorylcholine
-
 ?
phosphatidylcholine + H2O
-
 Bacillus cereus 1,2-diacyl-sn-glycerol + phosphorylcholine
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 28500, SDS-PAGE Bacillus cereus

Synonyms

Synonyms Comment Organism
PLC
-
 Bacillus cereus
type C phospholipase
-
 Bacillus cereus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.9
-
 4-nitrophenylphosphorylcholine wild type enzyme, at pH 7.0 and 50°C Bacillus cereus
4.8
-
 4-nitrophenylphosphorylcholine mutant enzyme F66Y, at pH 7.0 and 50°C Bacillus cereus
5.4
-
 4-nitrophenylphosphorylcholine mutant enzyme F66W, at pH 7.0 and 50°C Bacillus cereus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
98
-
 4-nitrophenylphosphorylcholine wild type enzyme, at pH 7.0 and 50°C Bacillus cereus
276
-
 4-nitrophenylphosphorylcholine mutant enzyme F66Y, at pH 7.0 and 50°C Bacillus cereus
365
-
 4-nitrophenylphosphorylcholine mutant enzyme F66W, at pH 7.0 and 50°C Bacillus cereus