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Literature summary for 3.1.4.3 extracted from

  • Shimanouchi, T.; Kawasaki, H.; Fuse, M.; Umakoshi, H.; Kuboi, R.
    Membrane fusion mediated by phospholipase C under endosomal pH conditions (2013), Colloids Surf. B Biointerfaces, 103, 75-83.
    View publication on PubMed
Search for more literature for 3.1.4.3

Application

Application Comment Organism
pharmacology phospholipase C is considered to be one of key enzymes for the design of drug delivery system using the endocytosis route, because PLC can catalyze the membrane fusion between cell membranes and phospholipid vehicles (liposomes) Bacillus cereus

Inhibitors

Inhibitors Comment Organism Structure
additional information excess diacylglycerol at pH 7.5 at approximately 12% inhibits the membrane fusion activity of the enzyme Bacillus cereus
o-phenanthroline inhibits the phospholipase C hydrolytic activity of the enzyme, but not its membrane fusion activity Bacillus cereus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phosphatidylcholine + H2O Bacillus cereus
-
 1,2-sn-diacylglycerol + phosphocholine
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus cereus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation
-
 Bacillus cereus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information phospholipase C can catalyze the membrane fusion between cell membranes and phospholipid vehicles (liposomes) at pH 4.0-7.5, highest activity at pH 5.0, 37°C. The liposomes are composed of 1,2-dioleoyl-sn-glycero-3-phosphocholine, 1,2-dipalmitoyl-sn-glycero-3-phosphocholine, and cholesterol in a 1:1:1 molar ratio, membrane fusion is dependent of the lipid composition, overview. The enzyme takes a molten-globule state, with a large fluctuation at acidic pH, mechanism of enzyme-induced membrane fusion Bacillus cereus ?
-
 ?
phosphatidylcholine + H2O
-
 Bacillus cereus 1,2-sn-diacylglycerol + phosphocholine
-
 ?

Subunits

Subunits Comment Organism
More secondary and tertiary structure analysis of the enzyme at different pH values from pH 2.0-7.5, overview Bacillus cereus

Synonyms

Synonyms Comment Organism
PLC
-
 Bacillus cereus

General Information

General Information Comment Organism
additional information secondary and tertiary structure analysis of the enzyme at different pH values from pH 2.0-7.5, overview Bacillus cereus