Protein Variants | Comment | Organism |
---|---|---|
C28A/C29A/D30A | site-directed mutagenesis, the mutation has no effect on PC-PLC maturation | Listeria monocytogenes |
D30A/E31A | site-directed mutagenesis, the mutation decreases the efficacy of PC-PLC processing to 75% of wild-type level | Listeria monocytogenes |
E31A | site-directed mutagenesis, the mutation has no effect on PC-PLC maturation | Listeria monocytogenes |
E31A/Y32A/L33A | site-directed mutagenesis, the mutation decreases the efficacy of PC-PLC processing to 42% of wild-type level | Listeria monocytogenes |
L33A | site-directed mutagenesis, the mutation decreases the efficacy of PC-PLC processing to 54% of wild-type level | Listeria monocytogenes |
additional information | construction and generation of PC-PLC propeptide deletion mutants | Listeria monocytogenes |
P36A | site-directed mutagenesis, the mutation decreases the efficacy of PC-PLC processing to 82% of wild-type level | Listeria monocytogenes |
Q34A/T35A | site-directed mutagenesis, the mutation has no effect on PC-PLC maturation | Listeria monocytogenes |
Q34A/T35A/P36A | site-directed mutagenesis, the mutation decreases the efficacy of PC-PLC processing to 79% of wild-type level | Listeria monocytogenes |
Q34K/T35P | site-directed mutagenesis, the mutation has no effect on PC-PLC maturation | Listeria monocytogenes |
Y32A | site-directed mutagenesis, the mutation decreases the efficacy of PC-PLC processing to 68% of wild-type level | Listeria monocytogenes |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
additional information | phosphatidylcholine phospholipase C compartmentalization and enzymatic activity is regulated by a 24-amino-acid propeptide, Cys28-Ser51. During intracytosolic multiplication, bacteria accumulate the proform of PC-PLC at their membrane-cell-wall interface, whereas during cell-to-cell spread vacuolar acidification leads to maturation and rapid translocation of PCPLC across the cell wall in a manner that is dependent on Mpl, the metalloprotease of Listeria | Listeria monocytogenes | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Listeria monocytogenes | - |
several strains | - |
Synonyms | Comment | Organism |
---|---|---|
PC-PLC | - |
Listeria monocytogenes |
phosphatidylcholine phospholipase C | - |
Listeria monocytogenes |
General Information | Comment | Organism |
---|---|---|
additional information | PC-PLC maturation between bacterial plasma membrane and bacterial cell wall, translocation across the bacterial cell wall, and activity in the host cell, schematic overview. The N-terminus of the PC-PLC propeptide regulates the compartmentalization of PC-PLC. Enzyme enzymatic activity is regulated by a 24-amino-acid propeptide Cys28-Ser51. Individual amino acid residues within the N-terminus of the PC-PLC propeptide regulate Mpl-mediated maturation of PC-PLC. A single amino acid propeptide is sufficient to inhibit PC-PLC activity, whereas a six-residue propeptide is sufficient for Mpl to mediate the proteolytic maturation of PC-PLC | Listeria monocytogenes |