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Literature summary for 3.1.4.3 extracted from
- Cellular localization and functional role of phosphatidylcholine-specific phospholipase C in NK cells (2001), J. Immunol., 167, 2642-2650.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| lymphokine | activation by lymphokines and/or in lytic granule exocytosis | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| tricyclodecan-9-yl-xanthogenate | inhibition is associated with decrease of enzyme exposed on cell surface and accumulation of cytoplasmic enzyme in Golgi region; preincubation of NK cells with a PC-PLC inhibitor, tricyclodecan-9-yl-xanthogenate, strongly reduced NK-mediated cytotoxicity | Homo sapiens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | after activation by IL-2, cytoplasmic enzyme translocates to outer cell surface, mechanism | Homo sapiens | 5737 | - |
| cytoplasm | IL-2-dependent distribution between cytoplasm and ectoplasmic membrane surface in human NK cells | Homo sapiens | 5737 | - |
| ectoplasm | after activation by IL-2, cytoplasmic enzyme translocates to outer cell surface, mechanism | Homo sapiens | 43265 | - |
| endoplasmic reticulum | colocalization of PC-PLC with the ER, mainly within the Golgi apparatus in NK-resting cells. A much more extended colocalization of PC-PLC with the ER marker is observed in NK-activated cells, in which the enzyme is not exclusively confined to the Golgi region | Homo sapiens | 5783 | - |
| Golgi apparatus | colocalization of PC-PLC with the ER, mainly within the Golgi apparatus in NK-resting cells. A much more extended colocalization of PC-PLC with the ER marker is observed in NK-activated cells, in which the enzyme is not exclusively confined to the Golgi region | Homo sapiens | 5794 | - |
| membrane | ectoplasmic, IL-2-dependent distribution between cytoplasm and ectoplasmic membrane surface in human NK cells | Homo sapiens | 16020 | - |
| additional information | epifluorescence, CLSM, and flow cytometry experiments demonstrate that PC-PLC is expressed both in cytoplasmic compartments and on the outer cell surface of either resting or activated NK cells | Homo sapiens | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
two PC-PLC isoforms, 40 and 66 kDa. The same PC-PLC isoforms are also detected in some leukemia cells, such as K562 and DAUDI. In U937 cells only the 40 kDa isoform is detected | Homo sapiens |
| 66000 | - |
two PC-PLC isoforms, 40 and 66 kDa. The same PC-PLC isoforms are also detected in some leukemia cells, such as K562 and DAUDI | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
| Homo sapiens | - |
two isoforms | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| DAUDI cell | human lymphoblastoid leukemia cell line | Homo sapiens | - |
| K-562 cell | human erythroleukemia cell line | Homo sapiens | - |
| natural killer cell | - |
Homo sapiens | - |
| RPMI-8866 cell | EBV-transformed B cell line | Homo sapiens | - |
| U-937 cell | human monocytic leukemia cell line | Homo sapiens | - |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PC-PLC | - |
Homo sapiens |
| phosphatidylcholine-specific phospholipase C | - |
Homo sapiens |
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