Literature summary for 3.1.4.2 extracted from

Matsumoto, Y.; Kashiwabara, N.; Oyama, T.; Murayama, K.; Matsumoto, H.; Sakasegawa, S.; Sugimori, D.
Molecular cloning, heterologous expression, and enzymatic characterization of lysoplasmalogen-specific phospholipase D from Thermocrispum sp. (2016), FEBS Open Bio, 6, 1113-1130 .

Search for more literature for 3.1.4.2

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli Shuffle T7 cells	Thermocrispum sp. RD004668

Protein Variants

Protein Variants	Comment	Organism
A55D	the enzyme activity of the mutant is remarkably decreased compared with that of wild type enzyme	Thermocrispum sp. RD004668
A55N	the enzyme activity of the mutant is remarkably decreased compared with that of wild type enzyme	Thermocrispum sp. RD004668
E175A	inactive	Thermocrispum sp. RD004668
F211A	the enzyme activity of the mutant is remarkably decreased compared with that of wild type enzyme	Thermocrispum sp. RD004668
N56D	the enzyme activity of the mutant is remarkably decreased compared with that of wild type enzyme	Thermocrispum sp. RD004668
N56Q	the enzyme activity of the mutant is remarkably decreased compared with that of wild type enzyme	Thermocrispum sp. RD004668

Inhibitors

Inhibitors	Comment	Organism	Structure
1-stearoyl-2-hydroxy-sn-glycero-3-phosphate	89.8% residual activity at 4 mM	Thermocrispum sp. RD004668
Al3+	inhibited by more than 5 mM Al3+	Thermocrispum sp. RD004668
EDTA	complete inhibition at 2 mM	Thermocrispum sp. RD004668
L-alpha-lysophosphatidylcholine	60.7% residual activity at 4 mM	Thermocrispum sp. RD004668
L-alpha-lysophosphatidylethanolamine	50.9% residual activity at 4 mM	Thermocrispum sp. RD004668
Mg2+	5.4% residual activity at 2 mM	Thermocrispum sp. RD004668
additional information	glycerophosphocholine, CDP-choline, dithiothreitol, 2-mercaptoethanol, iodoacetamide, and phenylmethylsulfonyl fluoride have no effect on enzyme activity	Thermocrispum sp. RD004668
Triton X-100	about 30% residual activity at 0.1% (v/v)	Thermocrispum sp. RD004668

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0132	-	choline lysoplasmalogen	at pH 8.0 and 50°C	Thermocrispum sp. RD004668

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Al3+	the enzyme is activated in the presence of Al3+	Thermocrispum sp. RD004668
Ca2+	the enzyme is activated in the presence of 0.5-10 mM Ca2+	Thermocrispum sp. RD004668

Organism

Organism	UniProt	Comment	Textmining
Thermocrispum sp. RD004668	A0A0U4VTN7	-	-

Purification (Commentary)

Purification (Comment)	Organism
ammonium sulfate precipitation, Resource Iso column chromatography, Mono Q column chromatography, PPG-600M column chromatography, Giga Cap Q-650M column chromatography, and Superdex 200 gel filtration	Thermocrispum sp. RD004668

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
choline lysoplasmalogen + H2O	preferred substrate, i.e. 1-O-1'-(Z)-octadecenyl-2-hydroxy-sn-glycero-3-phosphocholine	Thermocrispum sp. RD004668	choline + 1-(1-alkenyl)-sn-glycero-3-phosphate	-	?
ethanolamine lysoplasmalogen + H2O	modest activity, i.e. 1-O-1'-(Z)-octadecenyl-2-hydroxy-sn-glycero-3-phosphoethanolamine	Thermocrispum sp. RD004668	ethanolamine + 1-(1-alkenyl)-sn-glycero-3-phosphate	-	?

Subunits

Subunits	Comment	Organism
?	x * 33452, calculated from amino acid sequence	Thermocrispum sp. RD004668

Synonyms

Synonyms	Comment	Organism
LyPls-PLD	-	Thermocrispum sp. RD004668
LyPls-specific PLD	-	Thermocrispum sp. RD004668
lysoplasmalogen-specific phospholipase D	-	Thermocrispum sp. RD004668

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
50	-	-	Thermocrispum sp. RD004668

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
4	37	the enzyme is stable between 4 and 37°C for 60 min at pH 8.0	Thermocrispum sp. RD004668

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
70.6	-	choline lysoplasmalogen	at pH 8.0 and 50°C	Thermocrispum sp. RD004668

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	-	Thermocrispum sp. RD004668

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
4.1	9.8	enzyme activity is maintained between pH 4.1 and 9.8 at 4°C for 4 h	Thermocrispum sp. RD004668

pI Value

Organism	Comment	pI Value Maximum	pI Value
Thermocrispum sp. RD004668	calculated from amino acid sequence	-	5.6

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)