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Literature summary for 3.1.4.2 extracted from
- A novel glycerophosphodiester phosphodiesterase, GDE5, controls skeletal muscle development via a non-enzymatic mechanism (2010), J. Biol. Chem., 285, 27652-27663.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of C-terminally His6-tagged enzyme in Spodoptera frugiperda Sf9 cells | Mus musculus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | GDE5 RNAi gene silencing results in an increase in intracellular sn-glycero-3-phosphocholine levels. Construction of a truncated GDE5 construct, GDE5DELTAC471, which contains a GP-PDE sequence identified in other GP-PDEs but lacks GroPCho phosphodiesterase activity, and it shows a similar inhibitory effect on myogenic differentiation like the full-length enzyme, overview | Mus musculus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cd2+ | - |
Mus musculus |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | activates, required | Mus musculus | |
| additional information | no activation by Ca2+ | Mus musculus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 81000 | - |
x * 81000, recombinant His6-tagged enzyme, SDS-PAGE | Mus musculus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sn-glycero-3-phosphocholine + H2O | Mus musculus | - |
choline + sn-glycerol 3-phosphate | - |
? | |
| sn-glycero-3-phosphocholine + H2O | Mus musculus C57BL/6 | - |
choline + sn-glycerol 3-phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | - |
- |
- |
| Mus musculus C57BL/6 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant C-terminally His6-tagged enzyme from Spodoptera frugiperda Sf9 cells by nickel affinity chromatography and gel filtration | Mus musculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| skeletal muscle | - |
Mus musculus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2 | - |
purified recombinant His-tagged GDE5, substrate is glycerophosphoethanolamine, pH 7.4, 37°C | Mus musculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glycerophosphoethanolamine + H2O | - |
Mus musculus | ? | - |
? | |
| glycerophosphoethanolamine + H2O | - |
Mus musculus C57BL/6 | ? | - |
? | |
| additional information | the enzyme shows no significant activity with glycerophosphoglycerol, GroPIns, and glycerophosphoserine as substrates | Mus musculus | ? | - |
? | |
| additional information | the enzyme shows no significant activity with glycerophosphoglycerol, GroPIns, and glycerophosphoserine as substrates | Mus musculus C57BL/6 | ? | - |
? | |
| sn-glycero-3-phosphocholine + H2O | - |
Mus musculus | choline + sn-glycerol 3-phosphate | - |
? | |
| sn-glycero-3-phosphocholine + H2O | - |
Mus musculus C57BL/6 | choline + sn-glycerol 3-phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 81000, recombinant His6-tagged enzyme, SDS-PAGE | Mus musculus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GDE5 | - |
Mus musculus |
| glycerophosphodiester phosphodiesterase | - |
Mus musculus |
| GP-PDE | - |
Mus musculus |
| More | GDE5 is an UgpQ-type GDE | Mus musculus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Mus musculus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Mus musculus |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | GDE5 expression is reduced in atrophied skeletal muscles in mice and decreasing GDE5 abundance promotes myoblastic differentiation, suggesting that decreased GDE5 expression has a counter-regulatory effect on the progression of skeletal muscleatrophy. Transgenic mice specifically overexpressing GDE5DELTAC471 in skeletal muscle have less type II fiber-rich skeletal muscle mass. Forced expression of full-length GDE5 in cultured myoblasts suppresses myogenic differentiation. Transgenic mice specifically expressing GDE5DELTAC471 in skeletal muscle show less skeletal muscle mass, especially type II fiber-rich muscle | Mus musculus |
| physiological function | GDE5 selectively hydrolyzes glycerophosphocholine and controls skeletal muscle development. GDE5 negatively regulates skeletal muscle development even without GroPCho phosphodiesterase activity | Mus musculus |
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