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Literature summary for 3.1.4.17 extracted from

  • Drexler, D.; Müller, M.; Rojas-Cordova, C.; Bandera, A.; Witte, G.
    Structural and biophysical analysis of the soluble DHH/DHHA1-type phosphodiesterase TM1595 from Thermotoga maritima (2017), Structure, 25, 1887-1897 .
    View publication on PubMed
Search for more literature for 3.1.4.17

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Thermotoga maritima

Crystallization (Commentary)

Crystallization (Comment) Organism
crystals of the enzyme are grown by hanging-drop vapor diffusion at 20°C after mixing 1 ml protein with 1 ml reservoir solution (0.1 M HEPES-NaOH pH 7.8, 25% w/v PEG4000, 0.2 M CaCl2) with a total reservoir volume of 300 ml. Crystals are cryo-protected by transfer into mother liquor supplemented with 25% v/v ethylene glycol prior to flash cooling in liquid nitrogen. Thermotoga maritima

Protein Variants

Protein Variants Comment Organism
D80N inactive mutant enzyme Thermotoga maritima

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.204
-
 5'-pApA pH 8.5, 23°C Thermotoga maritima
0.355
-
 5'-pApG pH 8.5, 23°C Thermotoga maritima

Organism

Organism UniProt Comment Textmining
Thermotoga maritima Q9X1T1
-
-
Thermotoga maritima DSM 3109 Q9X1T1
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Thermotoga maritima

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3',5'-cAMP + H2O the enzyme is preferentially degrading linear dinucleotides, such as 5'-pApA, 5'-pGpG, and 5'-pApG, compared with cyclic dinucleotide substrates Thermotoga maritima 5'-AMP
-
 ?
3',5'-cAMP + H2O the enzyme is preferentially degrading linear dinucleotides, such as 5'-pApA, 5'-pGpG, and 5'-pApG, compared with cyclic dinucleotide substrates Thermotoga maritima DSM 3109 5'-AMP
-
 ?
5'-pApA + H2O
-
 Thermotoga maritima 5'-AMP
-
 ?
5'-pApA + H2O
-
 Thermotoga maritima DSM 3109 5'-AMP
-
 ?
5'-pApG + H2O
-
 Thermotoga maritima 5'-AMP + 5'-GMP
-
 ?
5'-pApG + H2O
-
 Thermotoga maritima DSM 3109 5'-AMP + 5'-GMP
-
 ?
5'-pGpG + H2O
-
 Thermotoga maritima 5'-GMP
-
 ?
5'-pGpG + H2O
-
 Thermotoga maritima DSM 3109 5'-GMP
-
 ?
additional information the enzyme is preferentially degrading linear dinucleotides, such as 5'-pApA, 5'-pGpG, and 5'-pApG, compared with cyclic dinucleotide substrates Thermotoga maritima ?
-
 ?
additional information the enzyme is preferentially degrading linear dinucleotides, such as 5'-pApA, 5'-pGpG, and 5'-pApG, compared with cyclic dinucleotide substrates Thermotoga maritima DSM 3109 ?
-
 ?

Subunits

Subunits Comment Organism
dimer
-
 Thermotoga maritima

Synonyms

Synonyms Comment Organism
TM1595
-
 Thermotoga maritima

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.14
-
 5'-pApA pH 8.5, 23°C Thermotoga maritima
0.26
-
 5'-pApG pH 8.5, 23°C Thermotoga maritima