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Literature summary for 3.1.4.17 extracted from

  • Tian, Y.; Cui, W.; Huang, M.; Robinson, H.; Wan, Y.; Wang, Y.; Ke, H.
    Dual specificity and novel structural folding of yeast phosphodiesterase-1 for hydrolysis of second messengers cyclic adenosine and guanosine 3,5-monophosphate (2014), Biochemistry, 53, 4938-4945.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.4.17

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Saccharomyces cerevisiae

Crystallization (Commentary)

Crystallization (Comment) Organism
unliganded enzyme, and in complex with cGMP, both to 1.3 A resolution. For the massive interactions between two monomers, the dimeric form of the enzyme is a biologic unit for its function Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0773
-
 3',5'-cAMP pH 7.5, 22°C, presence of 1 mM EDTA Saccharomyces cerevisiae
0.0968
-
 3',5'-cGMP pH 7.5, 22°C, presence of 10 mM MgCl2 Saccharomyces cerevisiae
0.0988
-
 3',5'-cAMP pH 7.5, 22°C, presence of 10 mM MgCl2 Saccharomyces cerevisiae
0.1047
-
 3',5'-cGMP pH 7.5, 22°C Saccharomyces cerevisiae
0.1048
-
 3',5'-cGMP pH 7.5, 22°C, presence of 10 mM ZnCl2 Saccharomyces cerevisiae
0.11
-
 3',5'-cAMP pH 7.5, 22°C Saccharomyces cerevisiae
0.1157
-
 3',5'-cAMP pH 7.5, 22°C, presence of 10 mM ZnCl2 Saccharomyces cerevisiae
0.1175
-
 3',5'-cAMP pH 7.5, 22°C, presence of 10 mM MnCl2 Saccharomyces cerevisiae
0.1539
-
 3',5'-cGMP pH 7.5, 22°C, presence of 1 mM EDTA Saccharomyces cerevisiae
0.1913
-
 3',5'-cGMP pH 7.5, 22°C, presence of 10 mM MnCl2 Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae P22434
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3',5'-cAMP + H2O
-
 Saccharomyces cerevisiae 5'-AMP
-
 ?
3',5'-cGMP + H2O
-
 Saccharomyces cerevisiae 5'-GMP
-
 ?
additional information enzyxme displays dual specificity for hydrolysis of both cAMP and cGMP Saccharomyces cerevisiae ?
-
 ?

Synonyms

Synonyms Comment Organism
PDE1
-
 Saccharomyces cerevisiae
phosphodiesterase-1
-
 Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
10.8
-
 3',5'-cGMP pH 7.5, 22°C, presence of 10 mM ZnCl2 Saccharomyces cerevisiae
11.1
-
 3',5'-cAMP pH 7.5, 22°C, presence of 1 mM EDTA Saccharomyces cerevisiae
11.7
-
 3',5'-cGMP pH 7.5, 22°C, presence of 1 mM EDTA Saccharomyces cerevisiae
11.8
-
 3',5'-cGMP pH 7.5, 22°C Saccharomyces cerevisiae
11.8
-
 3',5'-cAMP pH 7.5, 22°C, presence of 10 mM MnCl2 Saccharomyces cerevisiae
14.3
-
 3',5'-cGMP pH 7.5, 22°C, presence of 10 mM MnCl2 Saccharomyces cerevisiae
15.1
-
 3',5'-cGMP pH 7.5, 22°C, presence of 10 mM MgCl2 Saccharomyces cerevisiae
16.9
-
 3',5'-cAMP pH 7.5, 22°C Saccharomyces cerevisiae
17.4
-
 3',5'-cAMP pH 7.5, 22°C, presence of 10 mM ZnCl2 Saccharomyces cerevisiae
21
-
 3',5'-cAMP pH 7.5, 22°C, presence of 10 mM MgCl2 Saccharomyces cerevisiae

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
75
-
 3',5'-cGMP pH 7.5, 22°C, presence of 10 mM MnCl2 Saccharomyces cerevisiae
76
-
 3',5'-cGMP pH 7.5, 22°C, presence of 1 mM EDTA Saccharomyces cerevisiae
100
-
 3',5'-cAMP pH 7.5, 22°C, presence of 10 mM MnCl2 Saccharomyces cerevisiae
103
-
 3',5'-cGMP pH 7.5, 22°C, presence of 10 mM ZnCl2 Saccharomyces cerevisiae
113
-
 3',5'-cGMP pH 7.5, 22°C Saccharomyces cerevisiae
144
-
 3',5'-cAMP pH 7.5, 22°C, presence of 1 mM EDTA Saccharomyces cerevisiae
150
-
 3',5'-cAMP pH 7.5, 22°C, presence of 10 mM ZnCl2 Saccharomyces cerevisiae
153
-
 3',5'-cAMP pH 7.5, 22°C Saccharomyces cerevisiae
156
-
 3',5'-cGMP pH 7.5, 22°C, presence of 10 mM MgCl2 Saccharomyces cerevisiae
213
-
 3',5'-cAMP pH 7.5, 22°C, presence of 10 mM MgCl2 Saccharomyces cerevisiae