Cloned (Comment) | Organism |
---|---|
expressed in a baculovirus/insect cell Sf9 system | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | amino acid residues N845, E866, E971, F972, and F1004 are involved in cAMP (substrate) binding, while Y751, H836, H840, E866, D950 and F1004 participate in cGMP (inhibitor) binding | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(Rp)-guanosine-3',5'-cyclic-S-(4-bromo-2,3-dioxobutyl)monophosphorothioate | time-dependent and irreversible inactivation of PDE3A | Homo sapiens | |
cGMP | competitive inhibitor of PDE3A acting directly at the catalytic site | Homo sapiens | |
additional information | NAD+ is not an inhibitor of PDE3A | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
nickel resin column chromatography | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
blood platelet | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3',5'-cAMP + H2O | 3',5'-cAMP is predominantly hydrolyzed by PDE3A | Homo sapiens | 5'-AMP | - |
? | |
3',5'-cGMP + H2O | - |
Homo sapiens | 5'-GMP | - |
? | |
additional information | NAD+ is not a substrate of PDE3A | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
cAMP phosphodiesterase | - |
Homo sapiens |
PDE3A | - |
Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0434 | - |
(Rp)-guanosine-3',5'-cyclic-S-(4-bromo-2,3-dioxobutyl)monophosphorothioate | in 45 mM HEPES (pH 7.2), 20 mM MgCl2, and 4 mM MES, at 25°C | Homo sapiens |