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Literature summary for 3.1.4.16 extracted from

  • Abel, S.; Nurnberger, T.; Ahnert, V.; Krauss, G.J.; Glund, K.
    Induction of an extracellular cyclic nucleotide phosphodiesterase as an accessory ribonucleolytic activity during phosphate starvation of cultured tomato cells (2000), Plant Physiol., 122, 543-552.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.4.16

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 5 mM, more than 90% inhibition Solanum lycopersicum
additional information not inhibited by 5 mM EDTA Solanum lycopersicum
Zn2+ 5 mM, more than 90% inhibition Solanum lycopersicum

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Solanum lycopersicum
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information independent of divalent metal ions, e.g. Mg2+, Mn2+, Ca2+ and Co2+, all at 5mM Solanum lycopersicum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
 1 * 70000, SDS-PAGE Solanum lycopersicum
75000
-
 gel filtration Solanum lycopersicum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
nucleoside 2',3'-cyclic phosphate + H2O Solanum lycopersicum RNA degradation, enzyme functions as an accessory ribonucleolytic activity that effectively hydrolyzes the primary products of RNase LE, a phosphate starvation-inducible ribonuclease generating 2’,3’-cyclic nucleoside monophosphates, to substrates for phosphate starvation-inducible phosphomonoesterases nucleoside 3'-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Solanum lycopersicum
-
 cv. Lukullus
-

Purification (Commentary)

Purification (Comment) Organism
from the spent culture medium of phosphate-starved cells, 15fold Solanum lycopersicum

Source Tissue

Source Tissue Comment Organism Textmining
cell suspension culture phosphate-starved cells Solanum lycopersicum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Solanum lycopersicum
100
-
 about, pH 6, 37°C, di(p-nitrophenyl) phosphate hydrolysis Solanum lycopersicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5'-dTMP p-nitrophenyl ester
-
 Solanum lycopersicum ?
-
 ?
cyclic 2',3'-AMP + H2O
-
 Solanum lycopersicum 3'-AMP only product ?
cyclic 2',3'-CMP + H2O
-
 Solanum lycopersicum 3'-CMP only product ?
cyclic 2',3'-GMP + H2O
-
 Solanum lycopersicum 3'-GMP only product ?
cyclic 2',3'-UMP + H2O
-
 Solanum lycopersicum 3'-UMP only product ?
cyclic 3',5'-AMP + H2O
-
 Solanum lycopersicum 3'-AMP mixture of 3’-AMP and 5’-AMP in a ratio of 6:1, no further hydrolysis to nucleoside and phosphate ?
cyclic 3',5'-CMP + H2O
-
 Solanum lycopersicum 3'-CMP mixture of 3’-CMP and 5’-CMP in a ratio of 6:1, no further hydrolysis to nucleoside and phosphate ?
cyclic 3',5'-GMP + H2O
-
 Solanum lycopersicum 3'-GMP mixture of 3’-GMP and 5’-GMP in a ratio of 6:1, no further hydrolysis to nucleoside and phosphate ?
cyclic 3',5'-UMP + H2O
-
 Solanum lycopersicum 3'-UMP mixture of 3’-UMP and 5’-UMP in a ratio of 6:1, no further hydrolysis to nucleoside and phosphate ?
di(p-nitrophenyl) phosphate + H2O synthetic substrate Solanum lycopersicum p-nitrophenol + p-nitrophenyl phosphate
-
 ?
nucleoside 2',3'-cyclic phosphate + H2O no apparent base specificity, 2fold higher specific activity with nucleoside 2',3'-cyclic phosphate than with the 3',5'-cyclic isomers Solanum lycopersicum nucleoside 3'-phosphate no further hydrolysis to nucleoside and phosphate ?
nucleoside 2',3'-cyclic phosphate + H2O RNA degradation, enzyme functions as an accessory ribonucleolytic activity that effectively hydrolyzes the primary products of RNase LE, a phosphate starvation-inducible ribonuclease generating 2’,3’-cyclic nucleoside monophosphates, to substrates for phosphate starvation-inducible phosphomonoesterases Solanum lycopersicum nucleoside 3'-phosphate
-
 ?
nucleoside 3',5'-cyclic phosphate + H2O no apparent base specificity, 2fold higher specific activity with nucleoside 2',3'-cyclic phosphate than with the 3',5'-cyclic isomers Solanum lycopersicum nucleoside 3'-phosphate mixture of nucleoside 3'-phosphate and nucleoside 5'-phosphate in a ratio of 6:1, no further hydrolysis to nucleoside and phosphate ?

Subunits

Subunits Comment Organism
monomer 1 * 70000, SDS-PAGE Solanum lycopersicum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Solanum lycopersicum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2
-
-
 Solanum lycopersicum

pI Value

Organism Comment pI Value Maximum pI Value
Solanum lycopersicum chromatofocusing
-
 8.1