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Literature summary for 3.1.4.16 extracted from

  • Anraku, Y.
    A new cyclic phosphodiesterase having a 3'-nucleotidase activity from Escherichia coli B. I. PURIFICATION AND SOME PROPERTIES OF THE ENZYME. (1964), J. Biol. Chem., 239, 3412-3419.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Zn2+
-
Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information
-
Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ activates Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli B / ATCC 11303
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Escherichia coli

Storage Stability

Storage Stability Organism
2-4°C, pH 7.4-7.6, Tris buffer, 20-30% loss of activity after 2 months Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cyclic 2',3'-AMP + H2O
-
Escherichia coli 3'-AMP
-
?
cyclic 2',3'-AMP + H2O
-
Escherichia coli B / ATCC 11303 3'-AMP
-
?
cyclic 2',3'-CMP + H2O
-
Escherichia coli 3'-CMP
-
?
cyclic 2',3'-CMP + H2O
-
Escherichia coli B / ATCC 11303 3'-CMP
-
?
cyclic 2',3'-GMP + H2O
-
Escherichia coli 3'-GMP
-
?
cyclic 2',3'-GMP + H2O
-
Escherichia coli B / ATCC 11303 3'-GMP
-
?
cyclic 2',3'-UMP + H2O
-
Escherichia coli 3'-UMP
-
?
cyclic 2',3'-UMP + H2O
-
Escherichia coli B / ATCC 11303 3'-UMP
-
?
di-p-nitrophenyl phosphate + H2O
-
Escherichia coli p-nitrophenol + p-nitrophenyl phosphate
-
?
di-p-nitrophenyl phosphate + H2O
-
Escherichia coli B / ATCC 11303 p-nitrophenol + p-nitrophenyl phosphate
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
inactivation Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 6.5 hydrolysis of di-p-nitrophenyl phosphate Escherichia coli
6.4
-
3'-nucleotidase activity and cyclic phosphodiesterase activity against cyclic 2',3'-UMP Escherichia coli