Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.4 | - |
bis-4-nitrophenyl phosphate | - |
Pseudomonas aeruginosa | |
1 | - |
2',3'-cAMP | - |
Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
- |
- |
Pseudomonas aeruginosa PAO-1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni2+-NTA resin column chromatography and Superdex-75 gel filtration | Pseudomonas aeruginosa |
Renatured (Comment) | Organism |
---|---|
- |
Pseudomonas aeruginosa |
Storage Stability | Organism |
---|---|
AcpH is stable enough that it can be concentrated and stored at concentrations up to 1 mg/ml without causing severe aggregation | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2',3'-cAMP + H2O | - |
Pseudomonas aeruginosa | 3'-AMP | - |
? | |
2',3'-cAMP + H2O | - |
Pseudomonas aeruginosa PAO-1 | 3'-AMP | - |
? | |
acyl-carrier protein + H2O | acyl-carrier protein holo-PA2966 is converted completely back to its apo-form within 40 min, whereas the acyl-carrier proteins PA1869 and PA4226 remain in its holo form even after prolonged incubation. Partial conversion is observed for acyl-carrier protein PA3334 with both the apo and holo forms present after the incubation | Pseudomonas aeruginosa | 4'-phosphopantetheine + apoprotein | - |
? | |
acyl-carrier protein + H2O | acyl-carrier protein holo-PA2966 is converted completely back to its apo-form within 40 min, whereas the acyl-carrier proteins PA1869 and PA4226 remain in its holo form even after prolonged incubation. Partial conversion is observed for acyl-carrier protein PA3334 with both the apo and holo forms present after the incubation | Pseudomonas aeruginosa PAO-1 | 4'-phosphopantetheine + apoprotein | - |
? | |
acyl-carrier protein domain of the iterative polyketide synthase in calicheamicin biosynthesis + H2O | partial conversion | Pseudomonas aeruginosa | ? | - |
? | |
acyl-carrier protein domain of the iterative polyketide synthase in calicheamicin biosynthesis + H2O | partial conversion | Pseudomonas aeruginosa PAO-1 | ? | - |
? | |
acyl-carrier protein from Escherichia coli fatty acid synthesis pathway + H2O | complete conversion | Pseudomonas aeruginosa | ? | - |
? | |
bis-4-nitrophenyl phosphate + H2O | - |
Pseudomonas aeruginosa | ? | - |
? | |
CalE8 + H2O | - |
Pseudomonas aeruginosa | ? | - |
? | |
additional information | AcpH does not hydrolyze 3',5'-cAMP | Pseudomonas aeruginosa | ? | - |
? | |
additional information | acyl-carrier protein domain from the modular polyketide synthase DEBS is a poor substrate for AcpH | Pseudomonas aeruginosa | ? | - |
? | |
additional information | AcpH does not hydrolyze 3',5'-cAMP | Pseudomonas aeruginosa PAO-1 | ? | - |
? | |
additional information | acyl-carrier protein domain from the modular polyketide synthase DEBS is a poor substrate for AcpH | Pseudomonas aeruginosa PAO-1 | ? | - |
? | |
peptidyl carrier protein domain of non-ribosomal peptide synthase + H2O | complete conversion | Pseudomonas aeruginosa | ? | - |
? | |
thymidine 5-monophosphate 4-nitrophenyl ester + H2O | - |
Pseudomonas aeruginosa | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | about half of the soluble fraction of AcpH is obtained as monomeric protein | Pseudomonas aeruginosa |
Synonyms | Comment | Organism |
---|---|---|
AcpH | - |
Pseudomonas aeruginosa |
acyl carrier protein phosphodiesterase | - |
Pseudomonas aeruginosa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
2',3'-cAMP | - |
Pseudomonas aeruginosa | |
0.21 | - |
bis-4-nitrophenyl phosphate | - |
Pseudomonas aeruginosa |