Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme with a a built-in N-terminal saposin domain does not require an external saposin activator protein | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
vapor diffusion method, using 0.2 M sodium acetate, 20% (w/v) polyethylene glycol 8000, 0.1 M sodium cacodylate, pH 6.0 | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D278A | inactive | Homo sapiens |
H319Y | inactive | Homo sapiens |
H425R | inactive | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
lysosome | - |
Homo sapiens | 5764 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | binuclear zinc center | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sphingomyelin + H2O | Homo sapiens | - |
ceramide + phosphocholine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P17405 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Homo sapiens |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, HiTrap phenyl column chromatography, Q-Sepharose column chromatography, and Superdex 200 gel filtration | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HEK-293 cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sphingomyelin + H2O | - |
Homo sapiens | ceramide + phosphocholine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
acid sphingomyelinase | - |
Homo sapiens |
ASM | - |
Homo sapiens |
SMPD1 | - |
Homo sapiens |