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Literature summary for 3.1.4.12 extracted from

  • Gorelik, A.; Liu, F.; Illes, K.; Nagar, B.
    Crystal structure of the human alkaline sphingomyelinase provides insights into substrate recognition (2017), J. Biol. Chem., 292, 7087-7094 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
additional information bile salts like taurocholate do not directly stimulate catalysis by the enzyme Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Sf9 insect cells Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with phosphocholine, hanging drop vapor diffusion method, using 20% (w/v) PEG 3350 as precipitant Homo sapiens

Protein Variants

Protein Variants Comment Organism
Y109L the mutation mutant decreases the hydrolysis rate of sphingomyelin and 4-nitrophenylphosphorylcholine 5-10fold compared to the wild type enzyme Homo sapiens
Y166L the mutation mutant decreases the hydrolysis rate of sphingomyelin and 4-nitrophenylphosphorylcholine 5-10fold compared to the wild type enzyme Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ contains 2 zinc ions Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sphingomyelin + H2O Homo sapiens
-
ceramide + phosphocholine
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q6UWV6
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA resin column chromatography and Superdex 200 gel filtration Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphorylcholine + H2O
-
Homo sapiens 4-nitrophenol + phosphorylcholine
-
?
bis(4-nitrophenyl)phosphate + H2O
-
Homo sapiens ?
-
?
additional information no activity with 4-nitrophenyl-TMP Homo sapiens ?
-
?
sphingomyelin + H2O
-
Homo sapiens ceramide + phosphocholine
-
?

Synonyms

Synonyms Comment Organism
alk-SMase
-
Homo sapiens
alkaline sphingomyelinase
-
Homo sapiens
ENPP7
-
Homo sapiens
NPP7
-
Homo sapiens