Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | bile salts like taurocholate do not directly stimulate catalysis by the enzyme | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expressed in Sf9 insect cells | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
in complex with phosphocholine, hanging drop vapor diffusion method, using 20% (w/v) PEG 3350 as precipitant | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
Y109L | the mutation mutant decreases the hydrolysis rate of sphingomyelin and 4-nitrophenylphosphorylcholine 5-10fold compared to the wild type enzyme | Homo sapiens |
Y166L | the mutation mutant decreases the hydrolysis rate of sphingomyelin and 4-nitrophenylphosphorylcholine 5-10fold compared to the wild type enzyme | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | contains 2 zinc ions | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sphingomyelin + H2O | Homo sapiens | - |
ceramide + phosphocholine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q6UWV6 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA resin column chromatography and Superdex 200 gel filtration | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl phosphorylcholine + H2O | - |
Homo sapiens | 4-nitrophenol + phosphorylcholine | - |
? | |
bis(4-nitrophenyl)phosphate + H2O | - |
Homo sapiens | ? | - |
? | |
additional information | no activity with 4-nitrophenyl-TMP | Homo sapiens | ? | - |
? | |
sphingomyelin + H2O | - |
Homo sapiens | ceramide + phosphocholine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
alk-SMase | - |
Homo sapiens |
alkaline sphingomyelinase | - |
Homo sapiens |
ENPP7 | - |
Homo sapiens |
NPP7 | - |
Homo sapiens |