Cloned (Comment) | Organism |
---|---|
expressed in Sf9 cells and RAW264.7 cells | Mus musculus |
Crystallization (Comment) | Organism |
---|---|
apo-enzyme and in complex with phosphorylcholine, hanging drop vapor diffusion method, using 0.2 M NH4NO3 and 25% (w/v) PEG 3350 | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
H135A | almost inactive | Mus musculus |
K140M | the mutant shows reduced activity compared to the wild type enzyme | Mus musculus |
K140M/N141A | almost inactive | Mus musculus |
N141A | the mutant shows reduced activity compared to the wild type enzyme | Mus musculus |
N200A | the mutant shows increased activity compared to the wild type enzyme | Mus musculus |
Y198F | the mutant shows reduced activity compared to the wild type enzyme | Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell surface | - |
Mus musculus | 9986 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | contains two Zn2+ ions | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | P58242 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + H2O | - |
Mus musculus | AMP + phosphate | - |
? | |
ATP + H2O | - |
Mus musculus | ADP + phosphate | - |
? | |
bis(4-nitrophenyl)phosphate + H2O | - |
Mus musculus | ? | - |
? | |
CDP-choline + H2O | - |
Mus musculus | CMP + phosphorylcholine | - |
? | |
additional information | no activity with AMP and diadenosine tetraphosphate | Mus musculus | ? | - |
? | |
additional information | the soluble enzyme form that lacks the GPI moiety responsible for anchoring SMPDL3B to the cell surface displays no head group hydrolysis with the following substrates: sphingomyelin in Triton X-100 micelles, sphingomyelin in neutral liposomes (also comprising phosphatidylcholine and cholesterol), the soluble micelle-forming lipids sphingosylphosphorylcholine, and platelet-activating factor | Mus musculus | ? | - |
? | |
sphingomyelin + H2O | - |
Mus musculus | ceramide + phosphorylcholine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
acid sphingomyelinase-like phosphodiesterase 3B | - |
Mus musculus |
SMPDL3B | - |
Mus musculus |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme acts as a negative regulator of innate immune signaling, affecting cellular lipid composition and membrane fluidity | Mus musculus |