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Literature summary for 3.1.4.12 extracted from
- Identification and characterization of Laodelphax striatellus (Insecta Hemiptera Delphacidae) neutral sphingomyelinase (2017), Insect Mol. Biol., 26, 392-402 .
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| dithiothreitol | - |
Laodelphax striatellus |  |
| EGTA | stimulates activity at low concentrations | Laodelphax striatellus | |
| additional information | phospholipids like cardiolipin, phosphatidic acid, phosphatidylcholine, phosphatidylethanolamine, and phosphatidylserine do not stimulate enzyme activity | Laodelphax striatellus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in High Five insect cells | Laodelphax striatellus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | significant inhibition at 5 mM | Laodelphax striatellus | |
| GW4869 | - |
Laodelphax striatellus | |
| Zn2+ | complete inhibition at 0.1 mM | Laodelphax striatellus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| endoplasmic reticulum | - |
Laodelphax striatellus | 5783 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required, high concentrations of up to 50 mM of Mn2+ stimulate enzyme activity | Laodelphax striatellus | |
| Mn2+ | required, low concentrations of Mn2+ stimulate enzyme activity | Laodelphax striatellus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sphingomyelin + H2O | Laodelphax striatellus | - |
ceramide + phosphorylcholine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Laodelphax striatellus | A0A2U6TC85 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| head | - |
Laodelphax striatellus | - |
| leg | - |
Laodelphax striatellus | - |
| midgut | - |
Laodelphax striatellus | - |
| ovary | highest expression | Laodelphax striatellus | - |
| spermatheca | - |
Laodelphax striatellus | - |
| wing | - |
Laodelphax striatellus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-[[6-[(7-nitro-2-1,3-benzoxadiazol-4-yl)amino]hexanoyl]-hexanoyl]-sphingosine-1-phosphocholine + H2O | - |
Laodelphax striatellus | N-[6-[(7-nitro-2-1,3-benzoxadiazol-4-yl)amino]hexanoyl]-D-erythro-sphingosine + phosphorylcholine | - |
? | |
| sphingomyelin + H2O | - |
Laodelphax striatellus | ceramide + phosphorylcholine | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| neutral sphingomyelinase | - |
Laodelphax striatellus |
| nSMase | - |
Laodelphax striatellus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
at pH 8.0 | Laodelphax striatellus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 27 | 40 | the enzyme shows more than 60% activity between 27 and 40°C, and about 40% activity at 45°C | Laodelphax striatellus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Laodelphax striatellus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
the enzyme shows 50% activity at pH 7.5 and 80% activity at pH 9.0 | Laodelphax striatellus |
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