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Literature summary for 3.1.4.12 extracted from

  • Lim, S.M.; Yeung, K.; Tresaugues, L.; Ling, T.H.; Nordlund, P.
    The structure and catalytic mechanism of human sphingomyelin phosphodiesterase like 3a--an acid sphingomyelinase homologue with a novel nucleotide hydrolase activity (2016), FEBS J., 283, 1107-1123 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Sf9 insect cells Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
catalytic domain in complex with CMP, sitting drop vapor diffusion method, using 2.08 M disodium malonate pH 5, 0.23 M sodium thiocyanate Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Zn2+ high millimolar Zn2+ concentration leads to inhibition of enzyme activity Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.262
-
CDP-choline at pH 5.0 and 37°C Homo sapiens
0.306
-
ADP at pH 5.0 and 37°C Homo sapiens
0.327
-
ATP at pH 5.0 and 37°C Homo sapiens
0.348
-
ADP-ribose at pH 5.0 and 37°C Homo sapiens
0.391
-
CDP-ethanolamine at pH 5.0 and 37°C Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ contains two zinc ions Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q92484
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein the enzyme has seven sites for glycosylation Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and Superdex 200 gel filtration Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + H2O
-
Homo sapiens ?
-
?
ADP-ribose + H2O
-
Homo sapiens ?
-
?
ATP + H2O
-
Homo sapiens ?
-
?
CDP-choline + H2O
-
Homo sapiens CMP + phosphocholine
-
?
CDP-ethanolamine + H2O
-
Homo sapiens CMP + phosphoethanolamine
-
?
additional information no activity with sphingomyelin or AMP Homo sapiens ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 59400, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
SMPDL3A
-
Homo sapiens
sphingomyelin phosphodiesterase like 3a
-
Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.374
-
ADP at pH 5.0 and 37°C Homo sapiens
0.7357
-
ATP at pH 5.0 and 37°C Homo sapiens
1.29
-
CDP-choline at pH 5.0 and 37°C Homo sapiens
1.864
-
CDP-ethanolamine at pH 5.0 and 37°C Homo sapiens
2.07
-
ADP-ribose at pH 5.0 and 37°C Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.23
-
ADP at pH 5.0 and 37°C Homo sapiens
2.26
-
ATP at pH 5.0 and 37°C Homo sapiens
4.89
-
CDP-choline at pH 5.0 and 37°C Homo sapiens
5.71
-
CDP-ethanolamine at pH 5.0 and 37°C Homo sapiens
5.96
-
ADP-ribose at pH 5.0 and 37°C Homo sapiens