Cloned (Comment) | Organism |
---|---|
expressed in Sf9 insect cells | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
catalytic domain in complex with CMP, sitting drop vapor diffusion method, using 2.08 M disodium malonate pH 5, 0.23 M sodium thiocyanate | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | high millimolar Zn2+ concentration leads to inhibition of enzyme activity | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.262 | - |
CDP-choline | at pH 5.0 and 37°C | Homo sapiens | |
0.306 | - |
ADP | at pH 5.0 and 37°C | Homo sapiens | |
0.327 | - |
ATP | at pH 5.0 and 37°C | Homo sapiens | |
0.348 | - |
ADP-ribose | at pH 5.0 and 37°C | Homo sapiens | |
0.391 | - |
CDP-ethanolamine | at pH 5.0 and 37°C | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | contains two zinc ions | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q92484 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | the enzyme has seven sites for glycosylation | Homo sapiens |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography and Superdex 200 gel filtration | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + H2O | - |
Homo sapiens | ? | - |
? | |
ADP-ribose + H2O | - |
Homo sapiens | ? | - |
? | |
ATP + H2O | - |
Homo sapiens | ? | - |
? | |
CDP-choline + H2O | - |
Homo sapiens | CMP + phosphocholine | - |
? | |
CDP-ethanolamine + H2O | - |
Homo sapiens | CMP + phosphoethanolamine | - |
? | |
additional information | no activity with sphingomyelin or AMP | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 59400, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
SMPDL3A | - |
Homo sapiens |
sphingomyelin phosphodiesterase like 3a | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.374 | - |
ADP | at pH 5.0 and 37°C | Homo sapiens | |
0.7357 | - |
ATP | at pH 5.0 and 37°C | Homo sapiens | |
1.29 | - |
CDP-choline | at pH 5.0 and 37°C | Homo sapiens | |
1.864 | - |
CDP-ethanolamine | at pH 5.0 and 37°C | Homo sapiens | |
2.07 | - |
ADP-ribose | at pH 5.0 and 37°C | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.23 | - |
ADP | at pH 5.0 and 37°C | Homo sapiens | |
2.26 | - |
ATP | at pH 5.0 and 37°C | Homo sapiens | |
4.89 | - |
CDP-choline | at pH 5.0 and 37°C | Homo sapiens | |
5.71 | - |
CDP-ethanolamine | at pH 5.0 and 37°C | Homo sapiens | |
5.96 | - |
ADP-ribose | at pH 5.0 and 37°C | Homo sapiens |