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Literature summary for 3.1.4.12 extracted from

  • Muehle, C.; Huttner, H.B.; Walter, S.; Reichel, M.; Canneva, F.; Lewczuk, P.; Gulbins, E.; Kornhuber, J.
    Characterization of acid sphingomyelinase activity in human cerebrospinal fluid (2013), PLoS ONE, 8, e62912.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
diagnostics the extracellular enzyme from cerebrospinal fluid might hava a value as a potential biomarker for disease type, severity, progress or therapeutic success Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Zn2+ required for activity, best at 0.5-1 mM, inhibitory above 10 mM Homo sapiens
Zn2+ required for activity, best at 0.5-1 mM, inhibitory above 10 mM Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Michaelis-Menten kinetics Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular the enzyme is secreted Mus musculus
-
-
extracellular the enzyme is secreted Homo sapiens
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ required for activity, best at 0.5-1 mM, inhibitory above 10 mM Mus musculus
Zn2+ required for activity, best at 0.5-1 mM, inhibitory above 10 mM Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
a sphingomyelin + H2O Mus musculus
-
a ceramide + phosphocholine a ceramide is an N-acylsphingosine ?
a sphingomyelin + H2O Homo sapiens
-
a ceramide + phosphocholine a ceramide is an N-acylsphingosine ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
gene SMPD1
-
Mus musculus
-
gene SMPD1
-

Source Tissue

Source Tissue Comment Organism Textmining
cerebrospinal fluid cerebrospinal fluid contains a detectable level of secretory enzyme, which is unrelated to serum enzyme activity, no influence of sex or age on enzyme activity in cerebrospinal fluid Mus musculus
-
cerebrospinal fluid cerebrospinal fluid contains a detectable level of secretory enzyme, which is unrelated to serum enzyme activity, no influence of sex or age on enzyme activity in cerebrospinal fluid Homo sapiens
-

Storage Stability

Storage Stability Organism
22°C, storage of very small volume cerebrospinal fluid aliquots, below 0.02 ml, at room temperature or above lead to the rapid loss of enzyme activity Homo sapiens
the enzyme keeps over 80% activity after 8 rounds of freezing and thawing Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
a sphingomyelin + H2O
-
Mus musculus a ceramide + phosphocholine a ceramide is an N-acylsphingosine ?
a sphingomyelin + H2O
-
Homo sapiens a ceramide + phosphocholine a ceramide is an N-acylsphingosine ?
a sphingomyelin + H2O using the fluorescent substrate BODIPY-FL-C12-SM, i.e. N-(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-dodecanoyl)sphingosyl phosphocholine, D-7711 Homo sapiens a ceramide + phosphocholine a ceramide is an N-acylsphingosine ?
a sphingomyelin + H2O using the fluorescent substrate BODIPY-FL-C12-SM, N-(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-dodecanoyl)sphingosyl phosphocholine, or D-7711 Mus musculus a ceramide + phosphocholine a ceramide is an N-acylsphingosine ?
additional information establishment of a sensitive quantitative assay method using the fluorescent substrate BODIPY-FL-C12-SM, i.e. N-(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-dodecanoyl)sphingosyl phosphocholine, D-7711 Homo sapiens ?
-
?
additional information establishment of a sensitive quantitative assay method using the fluorescent substrate BODIPY-FL-C12-SM, N-(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-dodecanoyl)sphingosyl phosphocholine, or D-7711 Mus musculus ?
-
?

Synonyms

Synonyms Comment Organism
ASM
-
Mus musculus
ASM
-
Homo sapiens
sphingomyelinase
-
Mus musculus
sphingomyelinase
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
Mus musculus
37
-
-
Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
22
-
storage of very small volume cerebrospinal fluid aliquots, below 0.02 ml, at room temperature or above lead to the rapid loss of enzyme activity Homo sapiens
40
-
enzyme in cerebrospinal fluid, 2 h, stable under acidic conditions Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
assay at Mus musculus
5
-
assay at Homo sapiens

General Information

General Information Comment Organism
metabolism key enzyme in sphingolipid metabolism Mus musculus
metabolism key enzyme in sphingolipid metabolism Homo sapiens
physiological function as a key enzyme in sphingolipid metabolism, acid sphingomyelinase is involved in the regulation of cell fate and signaling via hydrolysis of sphingomyelin to form ceramide. Increased activity of the lysosomal form is associated with various pathological conditions Mus musculus
physiological function as a key enzyme in sphingolipid metabolism, acid sphingomyelinase is involved in the regulation of cell fate and signaling via hydrolysis of sphingomyelin to form ceramide. Increased activity of the lysosomal form is associated with various pathological conditions, the extracellular enzyme from cerebrospinal fluid migh also play a role in human disease Homo sapiens