Application | Comment | Organism |
---|---|---|
diagnostics | the extracellular enzyme from cerebrospinal fluid might hava a value as a potential biomarker for disease type, severity, progress or therapeutic success | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | required for activity, best at 0.5-1 mM, inhibitory above 10 mM | Homo sapiens | |
Zn2+ | required for activity, best at 0.5-1 mM, inhibitory above 10 mM | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | the enzyme is secreted | Mus musculus | - |
- |
extracellular | the enzyme is secreted | Homo sapiens | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | required for activity, best at 0.5-1 mM, inhibitory above 10 mM | Mus musculus | |
Zn2+ | required for activity, best at 0.5-1 mM, inhibitory above 10 mM | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
a sphingomyelin + H2O | Mus musculus | - |
a ceramide + phosphocholine | a ceramide is an N-acylsphingosine | ? | |
a sphingomyelin + H2O | Homo sapiens | - |
a ceramide + phosphocholine | a ceramide is an N-acylsphingosine | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
gene SMPD1 | - |
Mus musculus | - |
gene SMPD1 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cerebrospinal fluid | cerebrospinal fluid contains a detectable level of secretory enzyme, which is unrelated to serum enzyme activity, no influence of sex or age on enzyme activity in cerebrospinal fluid | Mus musculus | - |
cerebrospinal fluid | cerebrospinal fluid contains a detectable level of secretory enzyme, which is unrelated to serum enzyme activity, no influence of sex or age on enzyme activity in cerebrospinal fluid | Homo sapiens | - |
Storage Stability | Organism |
---|---|
22°C, storage of very small volume cerebrospinal fluid aliquots, below 0.02 ml, at room temperature or above lead to the rapid loss of enzyme activity | Homo sapiens |
the enzyme keeps over 80% activity after 8 rounds of freezing and thawing | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
a sphingomyelin + H2O | - |
Mus musculus | a ceramide + phosphocholine | a ceramide is an N-acylsphingosine | ? | |
a sphingomyelin + H2O | - |
Homo sapiens | a ceramide + phosphocholine | a ceramide is an N-acylsphingosine | ? | |
a sphingomyelin + H2O | using the fluorescent substrate BODIPY-FL-C12-SM, i.e. N-(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-dodecanoyl)sphingosyl phosphocholine, D-7711 | Homo sapiens | a ceramide + phosphocholine | a ceramide is an N-acylsphingosine | ? | |
a sphingomyelin + H2O | using the fluorescent substrate BODIPY-FL-C12-SM, N-(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-dodecanoyl)sphingosyl phosphocholine, or D-7711 | Mus musculus | a ceramide + phosphocholine | a ceramide is an N-acylsphingosine | ? | |
additional information | establishment of a sensitive quantitative assay method using the fluorescent substrate BODIPY-FL-C12-SM, i.e. N-(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-dodecanoyl)sphingosyl phosphocholine, D-7711 | Homo sapiens | ? | - |
? | |
additional information | establishment of a sensitive quantitative assay method using the fluorescent substrate BODIPY-FL-C12-SM, N-(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-dodecanoyl)sphingosyl phosphocholine, or D-7711 | Mus musculus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ASM | - |
Mus musculus |
ASM | - |
Homo sapiens |
sphingomyelinase | - |
Mus musculus |
sphingomyelinase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Mus musculus |
37 | - |
- |
Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
storage of very small volume cerebrospinal fluid aliquots, below 0.02 ml, at room temperature or above lead to the rapid loss of enzyme activity | Homo sapiens |
40 | - |
enzyme in cerebrospinal fluid, 2 h, stable under acidic conditions | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
assay at | Mus musculus |
5 | - |
assay at | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
metabolism | key enzyme in sphingolipid metabolism | Mus musculus |
metabolism | key enzyme in sphingolipid metabolism | Homo sapiens |
physiological function | as a key enzyme in sphingolipid metabolism, acid sphingomyelinase is involved in the regulation of cell fate and signaling via hydrolysis of sphingomyelin to form ceramide. Increased activity of the lysosomal form is associated with various pathological conditions | Mus musculus |
physiological function | as a key enzyme in sphingolipid metabolism, acid sphingomyelinase is involved in the regulation of cell fate and signaling via hydrolysis of sphingomyelin to form ceramide. Increased activity of the lysosomal form is associated with various pathological conditions, the extracellular enzyme from cerebrospinal fluid migh also play a role in human disease | Homo sapiens |