Cloned (Comment) | Organism |
---|---|
gene SMPD1, quantitative real-time PCR expression analysis, recombinant His-tagged enzyme expression in CHO cells and secretion to the culture medium | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | the enzyme is secreted | Homo sapiens | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | activates | Homo sapiens | |
Zn2+ | activates | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q8IUN0 | gene SMPD1 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | aSMase is an N-glycoprotein bearing 5 discrete asparagine-linked glycans with glycosylation of N395 and N520 being critical for normal secretion and function. SMPDL3A contains 7 potential N-linked glycosylation motifs (defined by the pattern NX[S|T], where X is any amino acid except proline). Notably, two of these motifs (N222 and N356) are perfectly aligned with the functionally important N395 and N520 N-linked sites of aSMase | Homo sapiens |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from CHO cell culture medium by nickel affinity chromatography | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
macrophage | - |
Homo sapiens | - |
monocyte | - |
Homo sapiens | - |
THP-1 cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
a sphingomyelin + H2O | - |
Homo sapiens | a ceramide + phosphocholine | i.e. an N-acylsphingosine | ? |
Synonyms | Comment | Organism |
---|---|---|
acid sphingomyelinase | - |
Homo sapiens |
aSMase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | 7.5 | assay at | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme is a member of the metallophosphoesterase enzyme family | Homo sapiens |
additional information | homology model of aSMase enzyme with consensus motif NX3CX3N, where X is any amino acid, overview. The central cysteine (C385) of the motif is also involved in a disulfide bridge with C431. The enzyme aSMase contains a sphingolipid activator protein (saposin) domain in its N-terminal region followed by a proline-rich linker, this domain also plays a role in stabilizing the structure of the enzyme | Homo sapiens |