Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.4.12 extracted from

  • Traini, M.; Quinn, C.M.; Sandoval, C.; Johansson, E.; Schroder, K.; Kockx, M.; Meikle, P.J.; Jessup, W.; Kritharides, L.
    Sphingomyelin phosphodiesterase-like 3A (SMPDL3A) is a novel nucleotide phosphodiesterase regulated by cholesterol in human macrophages (2014), J. Biol. Chem., 289, 32895-32913.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene SMPD1, quantitative real-time PCR expression analysis, recombinant His-tagged enzyme expression in CHO cells and secretion to the culture medium Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular the enzyme is secreted Homo sapiens
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ activates Homo sapiens
Zn2+ activates Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q8IUN0 gene SMPD1
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein aSMase is an N-glycoprotein bearing 5 discrete asparagine-linked glycans with glycosylation of N395 and N520 being critical for normal secretion and function. SMPDL3A contains 7 potential N-linked glycosylation motifs (defined by the pattern NX[S|T], where X is any amino acid except proline). Notably, two of these motifs (N222 and N356) are perfectly aligned with the functionally important N395 and N520 N-linked sites of aSMase Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from CHO cell culture medium by nickel affinity chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
macrophage
-
Homo sapiens
-
monocyte
-
Homo sapiens
-
THP-1 cell
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
a sphingomyelin + H2O
-
Homo sapiens a ceramide + phosphocholine i.e. an N-acylsphingosine ?

Synonyms

Synonyms Comment Organism
acid sphingomyelinase
-
Homo sapiens
aSMase
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 7.5 assay at Homo sapiens

General Information

General Information Comment Organism
evolution the enzyme is a member of the metallophosphoesterase enzyme family Homo sapiens
additional information homology model of aSMase enzyme with consensus motif NX3CX3N, where X is any amino acid, overview. The central cysteine (C385) of the motif is also involved in a disulfide bridge with C431. The enzyme aSMase contains a sphingolipid activator protein (saposin) domain in its N-terminal region followed by a proline-rich linker, this domain also plays a role in stabilizing the structure of the enzyme Homo sapiens