Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.4.12 extracted from

  • Perrotta, C.; Clementi, E.
    Biological roles of Acid and neutral sphingomyelinases and their regulation by nitric oxide (2010), Physiology, 25, 64-71.
    View publication on PubMed

Localization

Localization Comment Organism GeneOntology No. Textmining
lysosome lysosomal phosphodiesterase acid SMase Homo sapiens 5764
-
plasma membrane membrane-bound neutral SMase Homo sapiens 5886
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required by N-SMase Homo sapiens
Zn2+ required by the secreted enzyme form Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
x * 71000, N-SMase2, SDS-PAGE, x * 70000, mature A-SMase, SDS-PAGE, x * 75000, pre-pro-form of A-SMase, SDS-PAGE Homo sapiens
71000
-
x * 71000, N-SMase2, SDS-PAGE, x * 70000, mature A-SMase, SDS-PAGE, x * 75000, pre-pro-form of A-SMase, SDS-PAGE Homo sapiens
75000
-
x * 71000, N-SMase2, SDS-PAGE, x * 70000, mature A-SMase, SDS-PAGE, x * 75000, pre-pro-form of A-SMase, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
a sphingomyelin + H2O Homo sapiens
-
a ceramide + choline phosphate
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein the mature form and its precursors have six potential N-glycosylation sites, and at least five of them are important for proper folding, protection against proteolysis, and enzymatic activity of A-SMase. A-SMase has a mannose 6-phosphate residue that is required for lysosomal targetting of the enzyme via the mannose 6-phosphate receptor Homo sapiens
proteolytic modification the 75-kDa pre-proform of A-SMase, which is a pre-polypeptide with little if any enzymatic activity in the endoplasmic reticulum, is cleaved to an intermediate pro-form of 72 kDa, and further to the mature enzyme of 70 kDa in the lysosomes Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
astrocyte cortical astrocyte, acid SMase Homo sapiens
-
brain brain-specific N-SMase2 Homo sapiens
-
microglia acid SMase Homo sapiens
-
T-lymphocyte
-
Homo sapiens
-
U-937 cell
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
a sphingomyelin + H2O
-
Homo sapiens a ceramide + choline phosphate
-
?

Subunits

Subunits Comment Organism
? x * 71000, N-SMase2, SDS-PAGE, x * 70000, mature A-SMase, SDS-PAGE, x * 75000, pre-pro-form of A-SMase, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
A-SMase
-
Homo sapiens
acid SMase
-
Homo sapiens
acid sphingomyelinase
-
Homo sapiens
N-SMase
-
Homo sapiens
neutral SMase
-
Homo sapiens
neutral sphingomyelinase
-
Homo sapiens

General Information

General Information Comment Organism
malfunction deficiency of the enzyme leads to lysosomal accumulation of sphingomyelin resulting in in Niemann-Pick types A and B diseases Homo sapiens
metabolism interaction in the NO/NOS and SMases/ceramide signaling pathways, overview Homo sapiens
additional information from the gene of A-SMase, another product may be generated, i.e., the secretory SMase, which is very similar to A-SMase, differing only in the intracellular trafficking and in the need of zinc for activation Homo sapiens
physiological function role for A-SMase in the release of plasma membrane-derived microparticles, overview A-SMase is a cation-independent hydrolase contributing to the catabolism of sphingomyelin in lysosomes. Stress is believed to activate A-SMase to generate ceramide, which serves as a cellular mediator in initiating apoptotic response, the process is controlled by NO, detailed overview. A-SMase activity is also crucial for developmental programmed cell death of oocytes. Modeling of A-SMase function in apoptosis, overview Homo sapiens