Application | Comment | Organism |
---|---|---|
drug development | TbnSMase is therefore genetically validated as a drug target against African trypanosomes, and suggests that interfering with the endocytic transport of variant surface glycoprotein is a highly desirable strategy for drug development against African trypanosomasis | Trypanosoma brucei |
Cloned (Comment) | Organism |
---|---|
nSMase overexpression as GST-tagged enzyme in Escherichia coli strain C43 (DE3) | Trypanosoma brucei |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Trypanosoma brucei | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Trypanosoma brucei |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
81000 | - |
x * 81000, recombinant GST-tagged nSMase, SDS-PAGE | Trypanosoma brucei |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
a sphingomyelin + H2O | Trypanosoma brucei | - |
a ceramide + choline phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma brucei | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
a sphingomyelin + H2O | - |
Trypanosoma brucei | a ceramide + choline phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 81000, recombinant GST-tagged nSMase, SDS-PAGE | Trypanosoma brucei |
Synonyms | Comment | Organism |
---|---|---|
neutral sphingomyelinase | - |
Trypanosoma brucei |
General Information | Comment | Organism |
---|---|---|
malfunction | loss of nSMase activity leads to a Big-Eye-like phenotype, and affect s protein trafficking, as well as VSG trafficking, overview | Trypanosoma brucei |
metabolism | the essential neutral sphingomyelinase is involved in the trafficking of the variant surface glycoprotein in the bloodstream form of Trypanosoma brucei | Trypanosoma brucei |
physiological function | the neutral sphingomyelinase is essential for growth and survival of the parasite. The formation of ceramide in the endoplasmic reticulum affects post-Golgi sorting and rate of deposition of newly synthesized GPI-anchored variant surface glycoprotein on the cell surface | Trypanosoma brucei |