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Literature summary for 3.1.4.12 extracted from

  • Wu, B.X.; Clarke, C.J.; Matmati, N.; Montefusco, D.; Bartke, N.; Hannun, Y.A.
    Identification of novel anionic phospholipid binding domains in neutral sphingomyelinase 2 with selective binding preference (2011), J. Biol. Chem., 286, 22362-22371.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
anionic phospholipids APLs, the enzymatic activity of nSMase2 is dependent on anionic phospholipids, structural requirements for APL-selective binding of nSMase2, overview. nSMase2 interacts specifically and directly with several APLs, including phosphatidylserine and phosphatidic acid. Identification of two discrete APL binding domains in the N terminus of nSMase2, the nSMase2 N-terminal domain is essential for nSMase interaction with APLs Mus musculus

Cloned(Commentary)

Cloned (Comment) Organism
expression of (E)GFP-fused wild-type nSMase2 and of several (E)GFP-fused truncated mutant nSMase2s possessing additional V5- or FLAG-tags in HEK-293 or MCF-7 cells Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
plasma membrane associated via anionic phospholipids binding sites at the enzyme's N-terminus Mus musculus 5886
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Organism

Organism UniProt Comment Textmining
Mus musculus
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-
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Synonyms

Synonyms Comment Organism
neutral sphingomyelinase 2
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Mus musculus
nSMase2
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Mus musculus

General Information

General Information Comment Organism
physiological function APLs, the enzymatic activity of nSMase2 is dependent on anionic phospholipids, structural requirements for APL-selective binding of nSMase2, overview. nSMase2 interacts specifically and directly with several APLs, including phosphatidylserine and phosphatidic acid. Identification of two discrete APL binding domains in the N terminus of nSMase2, the nSMase2 N-terminal domain is essential for nSMase interaction with APLs. APL binding domains are important for nSMase2 to localize at the plasma membrane Mus musculus