Activating Compound | Comment | Organism | Structure |
---|---|---|---|
phosphatidylserine | - |
Bos taurus |
Cloned (Comment) | Organism |
---|---|
expressed in COS-7 cells | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
GW4869 | inhibited by 0.001-0.1 mM | Bos taurus | |
additional information | not inhibited by fumonisin B1 | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0203 | - |
sphingomyelin | in 100 mM Tris-HCl (pH 7.5), at 37°C | Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Bos taurus | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activated by micromolar concentrations of Ca2+ (0.0001 or 0.00001 mM) under Mg2+-free conditions, ceramide generation is dependent upon the calcium ionophore A23187, nSMase is not activated by Ca2+ cations in the presence of 1 mM Mg2+ | Bos taurus | |
additional information | Cu2+, Zn2+, and Ni2+ are unable to stimulate activity, nSMase is not activated by Ca2+ cations in the presence of 1 mM Mg2+ | Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
75000 | - |
SDS-PAGE | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, DEAE-cellulose column chromatography, butyl-Toyopearl column chromatography, DEAE-5PW HPLC column chromatography, Sephacryl S-300 gel filtration, Mono Q column chromatography, Heparin Hi-TRAP column chromatography, and Superose 12 gel filtration | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.000114 | - |
after 1fold purification, at 37°C | Bos taurus |
0.9334 | - |
after 8183.3fold purification, at 37°C | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sphingomyelin + H2O | - |
Bos taurus | N-acylsphingosine + choline phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
neutral sphingomyelinase 2 | - |
Bos taurus |
nSMase2 | - |
Bos taurus |