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Literature summary for 3.1.4.12 extracted from

  • Sugimori, D.
    Purification, characterization, and gene cloning of sphingomyelinase C from Streptomyces griseocarneus NBRC13471 (2009), J. Biosci. Bioeng., 108, 293-298.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information not affected by 6% sodium taurocholate and 6% sodium deoxycholate Streptomyces griseocarneus
sodium cholate 129% relative activity at 6% (v/v) sodium cholate Streptomyces griseocarneus
Triton X-100 205% relative activity at 0.1% (v/v) Triton X-100 Streptomyces griseocarneus

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli DH5alpha cells Streptomyces griseocarneus

Inhibitors

Inhibitors Comment Organism Structure
dithiothreitol 33.1% residual activity at 2 mM Streptomyces griseocarneus
EDTA complete inhibition at 2 mM Streptomyces griseocarneus
additional information the enzyme is not inhibited by mercaptoethanol and iodoacetamide Streptomyces griseocarneus
phenylmethylsulfonyl fluoride 70.3% residual activity at 2 mM Streptomyces griseocarneus
SDS 67.7% residual activity at 2 mM Streptomyces griseocarneus

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ 107% activity at 1 mM Mg2+ Streptomyces griseocarneus
Fe2+ 104% activity at 1 mM Mg2+ Streptomyces griseocarneus
Mg2+ required, 100% activity at 2.5-10 mM Mg2+ Streptomyces griseocarneus
Mn2+ required, 100% activity at 1 mM Mg2+ Streptomyces griseocarneus
additional information not stimulated by Ca2+, CO2+, and Fe3+ Streptomyces griseocarneus
additional information the order of potency to enhance the activity is Zn2+>Mn2+>Cu2+>Fe2+ Streptomyces griseocarneus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32102
-
1 * 32102, MALDI-TOF mass spectrometry Streptomyces griseocarneus
32130
-
calculated from amino acid sequence Streptomyces griseocarneus
38000
-
SDS-PAGE Streptomyces griseocarneus

Organism

Organism UniProt Comment Textmining
Streptomyces griseocarneus A6P7M9
-
-
Streptomyces griseocarneus NBRC13471 A6P7M9
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, DEAE-Sepharose column chromatography, HiTrap Q column chromatography, Resource Q column chromatography, and Superdex 200 gel filtration Streptomyces griseocarneus

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.246
-
culture supernatant, in 50 mM glycine-NaOH buffer (pH 9.6), at 37°C Streptomyces griseocarneus
98.4
-
400fold purified enzyme, in 50 mM glycine-NaOH buffer (pH 9.0), at 55°C Streptomyces griseocarneus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-di [(cis)-9-octadecenoyl]-sn-glycero-3-phospho-(1-rac-glycerol) + H2O 2.11% activity compared to sphingomyelin Streptomyces griseocarneus ?
-
?
1,2-di [(cis)-9-octadecenoyl]-sn-glycero-3-phospho-(1-rac-glycerol) + H2O 2.11% activity compared to sphingomyelin Streptomyces griseocarneus NBRC13471 ?
-
?
1,2-di(cis-9-octadecenoyl)-sn-glycerol-3-phosphate + H2O 9.04% activity compared to sphingomyelin Streptomyces griseocarneus ?
-
?
1,2-di(cis-9-octadecenoyl)-sn-glycerol-3-phosphate + H2O 9.04% activity compared to sphingomyelin Streptomyces griseocarneus NBRC13471 ?
-
?
1,2-dioleoyl-sn-glycero-3-phospho-L-serine + H2O 4.93% activity compared to sphingomyelin Streptomyces griseocarneus ?
-
?
1,2-dioleoyl-sn-glycero-3-phospho-L-serine + H2O 4.93% activity compared to sphingomyelin Streptomyces griseocarneus NBRC13471 ?
-
?
1,2-dioleoyl-sn-glycero-3-phosphocholine + H2O 0.7% activity compared to sphingomyelin Streptomyces griseocarneus ?
-
?
L-alpha-phosphatidylinositol + H2O 5.63% activity compared to sphingomyelin Streptomyces griseocarneus ?
-
?
additional information no activity towards 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine Streptomyces griseocarneus ?
-
?
additional information no activity towards 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine Streptomyces griseocarneus NBRC13471 ?
-
?
sphingomyelin + H2O 100% activity Streptomyces griseocarneus N-acylsphingosine + choline phosphate
-
?
sphingomyelin + H2O 100% activity Streptomyces griseocarneus NBRC13471 N-acylsphingosine + choline phosphate
-
?

Subunits

Subunits Comment Organism
monomer 1 * 32102, MALDI-TOF mass spectrometry Streptomyces griseocarneus

Synonyms

Synonyms Comment Organism
SMC
-
Streptomyces griseocarneus
sphingomyelinase C
-
Streptomyces griseocarneus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50 55
-
Streptomyces griseocarneus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 60
-
Streptomyces griseocarneus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4 60 the enzyme activity is stable between 4°C and 37°C for 30 min, activity drops abruptly above 37°C with less than 10% activity remaining at 50-60°C Streptomyces griseocarneus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
Streptomyces griseocarneus

pH Range

pH Minimum pH Maximum Comment Organism
7 11
-
Streptomyces griseocarneus

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 10 stable from pH 5.0-10.0, the enzyme retains activity between pH 5 and 10 for 30 min Streptomyces griseocarneus