Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 AI cells | Entamoeba histolytica |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | EDTA or EGTA in the reaction mixture have no effect on the basal activity | Entamoeba histolytica | |
scyphostatin | specific inhibitor of nSMase | Entamoeba histolytica | |
Zn2+ | nSMase activity of the recombinant protein nSM1 abolishes (12% residual activity) by 5 mM Zn2+ | Entamoeba histolytica |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Entamoeba histolytica | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | nSMase activity of the recombinant protein nSM1 increases 4.5fold in the presence of 0.5 mM Mn2+ | Entamoeba histolytica | |
additional information | Mg2+, Mn2+, Ca2+ and Zn2+, or Ca2+ have no significant effect on the activity | Entamoeba histolytica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
isoform nSM1, SDS-PAGE | Entamoeba histolytica |
37000 | - |
isoform nSM3, SDS-PAGE | Entamoeba histolytica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Entamoeba histolytica | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA agarose column chromatography, gel filtration | Entamoeba histolytica |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
trophozoite | - |
Entamoeba histolytica | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sphingomyelin + H2O | - |
Entamoeba histolytica | N-acylsphingosine + choline phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
neutral sphingomyelinase | - |
Entamoeba histolytica |
nSM1 | isoform | Entamoeba histolytica |
nSM3 | isoform | Entamoeba histolytica |
nSMase | - |
Entamoeba histolytica |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
2 | - |
in 100 mM Tris-HCl buffer (pH 7.5), at 37°C | Entamoeba histolytica | Zn2+ |