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Literature summary for 3.1.4.12 extracted from

  • Mendoza-Macias, C.L.; Barrios-Ceballos, M.P.; Anaya-Velazquez, F.; Nakada-Tsukui, K.; Nozaki, T.; Padilla-Vaca, F.
    Entamoeba histolytica: Molecular cloning and characterization of a novel neutral sphingomyelinase (2010), Exp. Parasitol., 125, 279-285.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 AI cells Entamoeba histolytica

Inhibitors

Inhibitors Comment Organism Structure
additional information EDTA or EGTA in the reaction mixture have no effect on the basal activity Entamoeba histolytica
scyphostatin specific inhibitor of nSMase Entamoeba histolytica
Zn2+ nSMase activity of the recombinant protein nSM1 abolishes (12% residual activity) by 5 mM Zn2+ Entamoeba histolytica

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Entamoeba histolytica 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ nSMase activity of the recombinant protein nSM1 increases 4.5fold in the presence of 0.5 mM Mn2+ Entamoeba histolytica
additional information Mg2+, Mn2+, Ca2+ and Zn2+, or Ca2+ have no significant effect on the activity Entamoeba histolytica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
isoform nSM1, SDS-PAGE Entamoeba histolytica
37000
-
isoform nSM3, SDS-PAGE Entamoeba histolytica

Organism

Organism UniProt Comment Textmining
Entamoeba histolytica
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography, gel filtration Entamoeba histolytica

Source Tissue

Source Tissue Comment Organism Textmining
trophozoite
-
Entamoeba histolytica
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sphingomyelin + H2O
-
Entamoeba histolytica N-acylsphingosine + choline phosphate
-
?

Synonyms

Synonyms Comment Organism
neutral sphingomyelinase
-
Entamoeba histolytica
nSM1 isoform Entamoeba histolytica
nSM3 isoform Entamoeba histolytica
nSMase
-
Entamoeba histolytica

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2
-
in 100 mM Tris-HCl buffer (pH 7.5), at 37°C Entamoeba histolytica Zn2+