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Literature summary for 3.1.4.12 extracted from

  • Yabu, T.; Imamura, S.; Yamashita, M.; Okazaki, T.
    Identification of Mg2+-dependent neutral sphingomyelinase 1 as a mediator of heat stress-induced ceramide generation and apoptosis (2008), J. Biol. Chem., 283, 29971-29982.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
additional information heat shock induces Mg2+-dependent neutral SMase activity in ZE cells as early as 1 h after exposure and reaches a peak of activity 2 h after heat shock Danio rerio

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3)pLysE cells Danio rerio

Protein Variants

Protein Variants Comment Organism
H272A mutant shows reduced activity Danio rerio

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum in the case of HEK293 transfectants, the zebrafish SMase 1 is also colocalized in both the Golgi apparatus and the endoplasmic reticulum Danio rerio 5783
-
Golgi apparatus SMase 1 is also localized in the Golgi apparatus Danio rerio 5794
-
microsome in zebrafish embryonic cells, the endogenous SMase enzyme is localized in the microsomal fraction Danio rerio
-
-
additional information no activity in cytosol Danio rerio
-
-
nucleus low activity in nuclear fractions Danio rerio 5634
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ dependent on Danio rerio

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46900
-
recombinant enzyme, putative molecular weight calculated from amino acid sequence and on SDS-PAGE Danio rerio

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Danio rerio neutral SMase 1 participates in an inducible ceramide-mediating, proapoptotic signaling pathway that operates in heat-induced apoptosis in zebrafish embryonic cells ?
-
?

Organism

Organism UniProt Comment Textmining
Danio rerio Q4LEU0
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap HP column chromatography and Sephacryl S-100 gel filtration Danio rerio

Source Tissue

Source Tissue Comment Organism Textmining
embryo
-
Danio rerio
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.000000025
-
recombinant enzyme, using (1-O-octadecyl-9,10-)lyso-platelet-activating factor as substrate, in 100 mM Tris-HCl, pH 7.5, 5 mM dithiothreitol, 5 mM MgCl2, at 37°C Danio rerio
0.00000045
-
recombinant enzyme, using sphingomyelin as substrate Danio rerio
0.0000264
-
wild type enzyme, using C6-7-nitro-2-1,3-benzoxadiazol-4-yl-sphingomyelin as substrate, in 100 mM Tris-HCl, pH 7.5, 5 mM dithiothreitol, 5 mM MgCl2, at 37°C Danio rerio
0.0000427
-
wild type enzyme after heat shock for 1 h at 37°C, using C6-7-nitro-2-1,3-benzoxadiazol-4-yl-sphingomyelin as substrate, in 100 mM Tris-HCl, pH 7.5, 5 mM dithiothreitol, 5 mM MgCl2, at 37°C Danio rerio
0.0000542
-
wild type enzyme after heat shock for 1 h at 38°C, using C6-7-nitro-2-1,3-benzoxadiazol-4-yl-sphingomyelin as substrate, in 100 mM Tris-HCl, pH 7.5, 5 mM dithiothreitol, 5 mM MgCl2, at 37°C Danio rerio

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-O-octadecyl-2-lyso-platelet-activating factor + H2O very little activity Danio rerio 1-O-octadecyl-sn-glycerol + phosphocholine
-
?
C6-7-nitro-2-1,3-benzoxadiazol-4-yl-sphingomyelin + H2O
-
Danio rerio ?
-
?
choline-methyl-sphingomyelin + H2O high activity Danio rerio ?
-
?
additional information neutral SMase 1 participates in an inducible ceramide-mediating, proapoptotic signaling pathway that operates in heat-induced apoptosis in zebrafish embryonic cells Danio rerio ?
-
?
additional information the enzyme has no activity against choline-methyl-phosphatidylcholine Danio rerio ?
-
?
sphingomyelin + H2O
-
Danio rerio N-acylsphingosine + choline phosphate
-
?

Synonyms

Synonyms Comment Organism
neutral SMase 1
-
Danio rerio
neutral sphingomyelinase 1 neutral SMase 1 acts as a mediator of stress-induced apoptosis Danio rerio

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
recombinant neutral SMase 1 hydrolyzes choline-methyl-sphingomyelin optimally at pH 7.5 in the presence of an Mg2+ ion Danio rerio