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Literature summary for 3.1.4.12 extracted from

  • Andersson, D.; Kotarsky, K.; Wu, J.; Agace, W.; Duan, R.D.
    Expression of alkaline sphingomyelinase in yeast cells and anti-inflammatory effects of the expressed enzyme in a rat colitis model (2008), Digest. Dis. Sci., 54, 1440-1448.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
taurocholate the enzyme activity is specifically stimulated by 6 mM taurocholate Homo sapiens
taurodeoxycholate the enzyme activity is specifically stimulated by about 2.5 mM taurodeoxycholate Homo sapiens

Application

Application Comment Organism
medicine intrarectal instillation of recombinant human Alk-SMase once daily for 1 week significantly reduces the inflammation score and protects the colonic epithelium from inflammatory destruction in a rat colitis model Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris strain GS115 Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Triton X-100 strong inhibition in the presence of 0.05-0.2% Triton X-100 Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
SE column chromatography, the enzyme is not retained in DEAE-Sepharose column equilibrated with 20 mM Tris-HCl buffer containing 0.075 M NaCl Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
intestine
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.027
-
recombinant human Alk-SMase, in 50 mM Tris Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sphingomyelin + H2O
-
Homo sapiens N-acylsphingosine + choline phosphate
-
?

Synonyms

Synonyms Comment Organism
alk-SMase key enzyme in the intestinal tract for digestion of dietary sphingomyelin Homo sapiens
alkaline sphingomyelinase
-
Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
7 10 the activity of Alk-SMase expressed from the yeast has a similar pH dependency, being low at acid pH, increasing after 7 and optimal at 9.0 Homo sapiens