Activating Compound | Comment | Organism | Structure |
---|---|---|---|
taurocholate | the enzyme activity is specifically stimulated by 6 mM taurocholate | Homo sapiens | |
taurodeoxycholate | the enzyme activity is specifically stimulated by about 2.5 mM taurodeoxycholate | Homo sapiens |
Application | Comment | Organism |
---|---|---|
medicine | intrarectal instillation of recombinant human Alk-SMase once daily for 1 week significantly reduces the inflammation score and protects the colonic epithelium from inflammatory destruction in a rat colitis model | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expressed in Pichia pastoris strain GS115 | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Triton X-100 | strong inhibition in the presence of 0.05-0.2% Triton X-100 | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
SDS-PAGE | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
SE column chromatography, the enzyme is not retained in DEAE-Sepharose column equilibrated with 20 mM Tris-HCl buffer containing 0.075 M NaCl | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
intestine | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.027 | - |
recombinant human Alk-SMase, in 50 mM Tris | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sphingomyelin + H2O | - |
Homo sapiens | N-acylsphingosine + choline phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
alk-SMase | key enzyme in the intestinal tract for digestion of dietary sphingomyelin | Homo sapiens |
alkaline sphingomyelinase | - |
Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 10 | the activity of Alk-SMase expressed from the yeast has a similar pH dependency, being low at acid pH, increasing after 7 and optimal at 9.0 | Homo sapiens |