Activating Compound | Comment | Organism | Structure |
---|---|---|---|
phosphatidylserine | - |
Homo sapiens | |
Triton X-100 | - |
Homo sapiens |
Cloned (Comment) | Organism |
---|---|
overexpression of V5-tagged wild-type and mutant nSMases and of GFP-tagged mutant C3A/C5A in MCF-7 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
C1A | site-directed mutagenesis, the altered residue shows altered palmitoylationin the mutant | Homo sapiens |
C2A | site-directed mutagenesis, the altered residue shows altered palmitoylationin the mutant | Homo sapiens |
C3A | site-directed mutagenesis, the altered residue shows altered palmitoylationin the mutant | Homo sapiens |
C3A/C392A | site-directed mutagenesis, the altered residues show altered palmitoylationin the mutant | Homo sapiens |
C3A/C395A | site-directed mutagenesis, the altered residues show altered palmitoylationin the mutant | Homo sapiens |
C3A/C395A/C396A | site-directed mutagenesis, the altered residues show altered palmitoylationin the mutant | Homo sapiens |
C3A/C396A C3A/C400A | site-directed mutagenesis, the altered residues show altered palmitoylationin the mutant | Homo sapiens |
C3A/C5A | site-directed mutagenesis, the altered residues show altered palmitoylationin the mutant | Homo sapiens |
C4A | site-directed mutagenesis, the altered residue shows altered palmitoylationin the mutant | Homo sapiens |
C5A | site-directed mutagenesis, the altered residue shows altered palmitoylationin the mutant | Homo sapiens |
C5A/C53A | site-directed mutagenesis, the altered residues show altered palmitoylationin the mutant | Homo sapiens |
C5A/C53A/C54A | site-directed mutagenesis, the altered residues show altered palmitoylationin the mutant | Homo sapiens |
C5A/C53A/C59A | site-directed mutagenesis, the altered residues show altered palmitoylationin the mutant | Homo sapiens |
C5A/C54A | site-directed mutagenesis, the altered residues show altered palmitoylationin the mutant | Homo sapiens |
C5A/C54A/C59A | site-directed mutagenesis, the altered residues show altered palmitoylationin the mutant | Homo sapiens |
C5A/C59A | site-directed mutagenesis, the altered residues show altered palmitoylationin the mutant | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | catalytic region of nSMase2 localizes to the cytosolic side of the plasma membrane, proposed membrane topology model, overview | Homo sapiens | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sphingomyelin + H2O | Homo sapiens | - |
N-acylsphingosine + choline phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
lipoprotein | neutral sphingomyelinase 2 is palmitoylated on multiple cysteine residues, palmitoylation of nSMase2 via thioester linkage, mutational analysis, overview | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
MCF-7 cell | mammary carcinoma cell line | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sphingomyelin + H2O | - |
Homo sapiens | N-acylsphingosine + choline phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
neutral sphingomyelinase 2 | - |
Homo sapiens |
nSMase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |