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Literature summary for 3.1.4.12 extracted from
- Neutral sphingomyelinase 2 is palmitoylated on multiple cysteine residues. Role of palmitoylation in subcellular localization (2007), J. Biol. Chem., 282, 10047-10056.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| phosphatidylserine | - |
Homo sapiens |  |
| Triton X-100 | - |
Homo sapiens | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression of V5-tagged wild-type and mutant nSMases and of GFP-tagged mutant C3A/C5A in MCF-7 cells | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C1A | site-directed mutagenesis, the altered residue shows altered palmitoylationin the mutant | Homo sapiens |
| C2A | site-directed mutagenesis, the altered residue shows altered palmitoylationin the mutant | Homo sapiens |
| C3A | site-directed mutagenesis, the altered residue shows altered palmitoylationin the mutant | Homo sapiens |
| C3A/C392A | site-directed mutagenesis, the altered residues show altered palmitoylationin the mutant | Homo sapiens |
| C3A/C395A | site-directed mutagenesis, the altered residues show altered palmitoylationin the mutant | Homo sapiens |
| C3A/C395A/C396A | site-directed mutagenesis, the altered residues show altered palmitoylationin the mutant | Homo sapiens |
| C3A/C396A C3A/C400A | site-directed mutagenesis, the altered residues show altered palmitoylationin the mutant | Homo sapiens |
| C3A/C5A | site-directed mutagenesis, the altered residues show altered palmitoylationin the mutant | Homo sapiens |
| C4A | site-directed mutagenesis, the altered residue shows altered palmitoylationin the mutant | Homo sapiens |
| C5A | site-directed mutagenesis, the altered residue shows altered palmitoylationin the mutant | Homo sapiens |
| C5A/C53A | site-directed mutagenesis, the altered residues show altered palmitoylationin the mutant | Homo sapiens |
| C5A/C53A/C54A | site-directed mutagenesis, the altered residues show altered palmitoylationin the mutant | Homo sapiens |
| C5A/C53A/C59A | site-directed mutagenesis, the altered residues show altered palmitoylationin the mutant | Homo sapiens |
| C5A/C54A | site-directed mutagenesis, the altered residues show altered palmitoylationin the mutant | Homo sapiens |
| C5A/C54A/C59A | site-directed mutagenesis, the altered residues show altered palmitoylationin the mutant | Homo sapiens |
| C5A/C59A | site-directed mutagenesis, the altered residues show altered palmitoylationin the mutant | Homo sapiens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | catalytic region of nSMase2 localizes to the cytosolic side of the plasma membrane, proposed membrane topology model, overview | Homo sapiens | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Homo sapiens | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sphingomyelin + H2O | Homo sapiens | - |
N-acylsphingosine + choline phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| lipoprotein | neutral sphingomyelinase 2 is palmitoylated on multiple cysteine residues, palmitoylation of nSMase2 via thioester linkage, mutational analysis, overview | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| MCF-7 cell | mammary carcinoma cell line | Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sphingomyelin + H2O | - |
Homo sapiens | N-acylsphingosine + choline phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| neutral sphingomyelinase 2 | - |
Homo sapiens |
| nSMase | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Homo sapiens |
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