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Literature summary for 3.1.4.12 extracted from

  • Clarke, C.J.; Hannun, Y.A.
    Neutral sphingomyelinases and nSMase2: bridging the gaps (2006), Biochim. Biophys. Acta, 1758, 1893-1901.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information nSMase2 is induced by TNFalpha, cytokines, cellular stresses such as UV-light, heat shock, apoptosis, and chemotherapeutic drugs, and by pathological stimuli such as amyloid-beta peptides and lipopolysaccharides Homo sapiens
protein kinase C-zeta mediates the activation of N-SMase by 1,25-dihydroxyvitamin D3, while protein kinase C-delta inhibits the activation Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
isozyme nSMase1 Homo sapiens
isozymes nSMase1 and nSMase2, overexpression of nSMase2 increases the shpingomyelin metabolism while overexpression of nSMase1 has no effect Mus musculus

Protein Variants

Protein Variants Comment Organism
additional information construction of nSMase2 and of nSMase1 knockout mice, the latter do not show lipid storage abnormalities nor deficient sphingomyelin metabolism, phenotype, overview Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
3-O-methylsphingomyelin
-
Bos taurus
3-O-methylsphingomyelin
-
Homo sapiens
3-O-methylsphingomyelin
-
Mus musculus
3-O-methylsphingomyelin
-
Rattus norvegicus
C11AG inhibits N-SMases in T-cells and macrophages Homo sapiens
GW4869 noncompetitive Homo sapiens
manumycin A
-
Homo sapiens
additional information protein kinase C-delta inhibits the activation of N-SMase by 1,25-dihydroxyvitamin D3 Homo sapiens
scyphostatin isolated from Discomycetes, specifically inhibits N-SMases Bos taurus
scyphostatin isolated from Discomycetes, specifically inhibits N-SMases Homo sapiens
scyphostatin isolated from Discomycetes, specifically inhibits N-SMases Mus musculus
scyphostatin isolated from Discomycetes, specifically inhibits N-SMases Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane nSMase2 is predominantly membrane-bound Homo sapiens 16020
-
plasma membrane
-
Mus musculus 5886
-
plasma membrane
-
Homo sapiens 5886
-
plasma membrane
-
Rattus norvegicus 5886
-
plasma membrane
-
Bos taurus 5886
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ nSMase2 is a Mg2+-dependent neutral sphingomyelinase Mus musculus
Mg2+ nSMase2 is a Mg2+-dependent neutral sphingomyelinase Homo sapiens
Mg2+ nSMase2 is a Mg2+-dependent neutral sphingomyelinase Rattus norvegicus
Mg2+ nSMase2 is a Mg2+-dependent neutral sphingomyelinase Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sphingomyelin + H2O Mus musculus nSMase2 is the key enzyme involved in apoptosis and stress-induced ceramide production by sphingomyelin hydrolysis, enzyme and ceramide signaling pathway regulation, the plasma membrane redox system is involved in regulation of the enzyme, overview N-acylsphingosine + choline phosphate
-
?
sphingomyelin + H2O Homo sapiens nSMase2 is the key enzyme involved in apoptosis and stress-induced ceramide production by sphingomyelin hydrolysis, enzyme and ceramide signaling pathway regulation, the plasma membrane redox system is involved in regulation of the enzyme, overview N-acylsphingosine + choline phosphate
-
?
sphingomyelin + H2O Rattus norvegicus nSMase2 is the key enzyme involved in apoptosis and stress-induced ceramide production by sphingomyelin hydrolysis, enzyme and ceramide signaling pathway regulation, the plasma membrane redox system is involved in regulation of the enzyme, overview N-acylsphingosine + choline phosphate
-
?
sphingomyelin + H2O Bos taurus nSMase2 is the key enzyme involved in apoptosis and stress-induced ceramide production by sphingomyelin hydrolysis, enzyme and ceramide signaling pathway regulation, the plasma membrane redox system is involved in regulation of the enzyme, overview N-acylsphingosine + choline phosphate
-
?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
multiple N-SMase isozymes, one of which is nSMase2
-
Homo sapiens
-
isozymes nSMase2 and nSMase1
-
Mus musculus
-
isozymes nSMase1 and nSMase2
-
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
nSMase2 and nSMase3, from brain Bos taurus
nSMase2, from brain Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
Rattus norvegicus
-
brain grey matter, high nSMase2 activity Homo sapiens
-
brain isozymes nSMase2 and nSMase3 Bos taurus
-
heart isozyme nSMase3 Bos taurus
-
HL-60 cell
-
Homo sapiens
-
liver
-
Homo sapiens
-
LNCaP cell
-
Homo sapiens
-
MCF-7 cell
-
Homo sapiens
-
mesangial cell
-
Rattus norvegicus
-
PC-12 cell
-
Rattus norvegicus
-
skeletal muscle isozyme nSMase3 Bos taurus
-
spleen
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sphingomyelin + H2O
-
Mus musculus N-acylsphingosine + choline phosphate
-
?
sphingomyelin + H2O
-
Homo sapiens N-acylsphingosine + choline phosphate
-
?
sphingomyelin + H2O
-
Rattus norvegicus N-acylsphingosine + choline phosphate
-
?
sphingomyelin + H2O
-
Bos taurus N-acylsphingosine + choline phosphate
-
?
sphingomyelin + H2O nSMase2 is the key enzyme involved in apoptosis and stress-induced ceramide production by sphingomyelin hydrolysis, enzyme and ceramide signaling pathway regulation, the plasma membrane redox system is involved in regulation of the enzyme, overview Mus musculus N-acylsphingosine + choline phosphate
-
?
sphingomyelin + H2O nSMase2 is the key enzyme involved in apoptosis and stress-induced ceramide production by sphingomyelin hydrolysis, enzyme and ceramide signaling pathway regulation, the plasma membrane redox system is involved in regulation of the enzyme, overview Homo sapiens N-acylsphingosine + choline phosphate
-
?
sphingomyelin + H2O nSMase2 is the key enzyme involved in apoptosis and stress-induced ceramide production by sphingomyelin hydrolysis, enzyme and ceramide signaling pathway regulation, the plasma membrane redox system is involved in regulation of the enzyme, overview Rattus norvegicus N-acylsphingosine + choline phosphate
-
?
sphingomyelin + H2O nSMase2 is the key enzyme involved in apoptosis and stress-induced ceramide production by sphingomyelin hydrolysis, enzyme and ceramide signaling pathway regulation, the plasma membrane redox system is involved in regulation of the enzyme, overview Bos taurus N-acylsphingosine + choline phosphate
-
?

Synonyms

Synonyms Comment Organism
N-SMase
-
Mus musculus
N-SMase
-
Homo sapiens
N-SMase
-
Rattus norvegicus
N-SMase
-
Bos taurus
neutral sphingomyelinase
-
Mus musculus
neutral sphingomyelinase
-
Homo sapiens
neutral sphingomyelinase
-
Rattus norvegicus
neutral sphingomyelinase
-
Bos taurus
nSMase2
-
Mus musculus
nSMase2
-
Homo sapiens
nSMase2
-
Rattus norvegicus
nSMase2
-
Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
nSMase2 Homo sapiens