Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | nSMase2 is induced by TNFalpha, cytokines, cellular stresses such as UV-light, heat shock, apoptosis, and chemotherapeutic drugs, and by pathological stimuli such as amyloid-beta peptides and lipopolysaccharides | Homo sapiens | |
protein kinase C-zeta | mediates the activation of N-SMase by 1,25-dihydroxyvitamin D3, while protein kinase C-delta inhibits the activation | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
isozyme nSMase1 | Homo sapiens |
isozymes nSMase1 and nSMase2, overexpression of nSMase2 increases the shpingomyelin metabolism while overexpression of nSMase1 has no effect | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of nSMase2 and of nSMase1 knockout mice, the latter do not show lipid storage abnormalities nor deficient sphingomyelin metabolism, phenotype, overview | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-O-methylsphingomyelin | - |
Bos taurus | |
3-O-methylsphingomyelin | - |
Homo sapiens | |
3-O-methylsphingomyelin | - |
Mus musculus | |
3-O-methylsphingomyelin | - |
Rattus norvegicus | |
C11AG | inhibits N-SMases in T-cells and macrophages | Homo sapiens | |
GW4869 | noncompetitive | Homo sapiens | |
manumycin A | - |
Homo sapiens | |
additional information | protein kinase C-delta inhibits the activation of N-SMase by 1,25-dihydroxyvitamin D3 | Homo sapiens | |
scyphostatin | isolated from Discomycetes, specifically inhibits N-SMases | Bos taurus | |
scyphostatin | isolated from Discomycetes, specifically inhibits N-SMases | Homo sapiens | |
scyphostatin | isolated from Discomycetes, specifically inhibits N-SMases | Mus musculus | |
scyphostatin | isolated from Discomycetes, specifically inhibits N-SMases | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | nSMase2 is predominantly membrane-bound | Homo sapiens | 16020 | - |
plasma membrane | - |
Mus musculus | 5886 | - |
plasma membrane | - |
Homo sapiens | 5886 | - |
plasma membrane | - |
Rattus norvegicus | 5886 | - |
plasma membrane | - |
Bos taurus | 5886 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | nSMase2 is a Mg2+-dependent neutral sphingomyelinase | Mus musculus | |
Mg2+ | nSMase2 is a Mg2+-dependent neutral sphingomyelinase | Homo sapiens | |
Mg2+ | nSMase2 is a Mg2+-dependent neutral sphingomyelinase | Rattus norvegicus | |
Mg2+ | nSMase2 is a Mg2+-dependent neutral sphingomyelinase | Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sphingomyelin + H2O | Mus musculus | nSMase2 is the key enzyme involved in apoptosis and stress-induced ceramide production by sphingomyelin hydrolysis, enzyme and ceramide signaling pathway regulation, the plasma membrane redox system is involved in regulation of the enzyme, overview | N-acylsphingosine + choline phosphate | - |
? | |
sphingomyelin + H2O | Homo sapiens | nSMase2 is the key enzyme involved in apoptosis and stress-induced ceramide production by sphingomyelin hydrolysis, enzyme and ceramide signaling pathway regulation, the plasma membrane redox system is involved in regulation of the enzyme, overview | N-acylsphingosine + choline phosphate | - |
? | |
sphingomyelin + H2O | Rattus norvegicus | nSMase2 is the key enzyme involved in apoptosis and stress-induced ceramide production by sphingomyelin hydrolysis, enzyme and ceramide signaling pathway regulation, the plasma membrane redox system is involved in regulation of the enzyme, overview | N-acylsphingosine + choline phosphate | - |
? | |
sphingomyelin + H2O | Bos taurus | nSMase2 is the key enzyme involved in apoptosis and stress-induced ceramide production by sphingomyelin hydrolysis, enzyme and ceramide signaling pathway regulation, the plasma membrane redox system is involved in regulation of the enzyme, overview | N-acylsphingosine + choline phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
multiple N-SMase isozymes, one of which is nSMase2 | - |
Homo sapiens | - |
isozymes nSMase2 and nSMase1 | - |
Mus musculus | - |
isozymes nSMase1 and nSMase2 | - |
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
nSMase2 and nSMase3, from brain | Bos taurus |
nSMase2, from brain | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Rattus norvegicus | - |
brain | grey matter, high nSMase2 activity | Homo sapiens | - |
brain | isozymes nSMase2 and nSMase3 | Bos taurus | - |
heart | isozyme nSMase3 | Bos taurus | - |
HL-60 cell | - |
Homo sapiens | - |
liver | - |
Homo sapiens | - |
LNCaP cell | - |
Homo sapiens | - |
MCF-7 cell | - |
Homo sapiens | - |
mesangial cell | - |
Rattus norvegicus | - |
PC-12 cell | - |
Rattus norvegicus | - |
skeletal muscle | isozyme nSMase3 | Bos taurus | - |
spleen | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sphingomyelin + H2O | - |
Mus musculus | N-acylsphingosine + choline phosphate | - |
? | |
sphingomyelin + H2O | - |
Homo sapiens | N-acylsphingosine + choline phosphate | - |
? | |
sphingomyelin + H2O | - |
Rattus norvegicus | N-acylsphingosine + choline phosphate | - |
? | |
sphingomyelin + H2O | - |
Bos taurus | N-acylsphingosine + choline phosphate | - |
? | |
sphingomyelin + H2O | nSMase2 is the key enzyme involved in apoptosis and stress-induced ceramide production by sphingomyelin hydrolysis, enzyme and ceramide signaling pathway regulation, the plasma membrane redox system is involved in regulation of the enzyme, overview | Mus musculus | N-acylsphingosine + choline phosphate | - |
? | |
sphingomyelin + H2O | nSMase2 is the key enzyme involved in apoptosis and stress-induced ceramide production by sphingomyelin hydrolysis, enzyme and ceramide signaling pathway regulation, the plasma membrane redox system is involved in regulation of the enzyme, overview | Homo sapiens | N-acylsphingosine + choline phosphate | - |
? | |
sphingomyelin + H2O | nSMase2 is the key enzyme involved in apoptosis and stress-induced ceramide production by sphingomyelin hydrolysis, enzyme and ceramide signaling pathway regulation, the plasma membrane redox system is involved in regulation of the enzyme, overview | Rattus norvegicus | N-acylsphingosine + choline phosphate | - |
? | |
sphingomyelin + H2O | nSMase2 is the key enzyme involved in apoptosis and stress-induced ceramide production by sphingomyelin hydrolysis, enzyme and ceramide signaling pathway regulation, the plasma membrane redox system is involved in regulation of the enzyme, overview | Bos taurus | N-acylsphingosine + choline phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
N-SMase | - |
Mus musculus |
N-SMase | - |
Homo sapiens |
N-SMase | - |
Rattus norvegicus |
N-SMase | - |
Bos taurus |
neutral sphingomyelinase | - |
Mus musculus |
neutral sphingomyelinase | - |
Homo sapiens |
neutral sphingomyelinase | - |
Rattus norvegicus |
neutral sphingomyelinase | - |
Bos taurus |
nSMase2 | - |
Mus musculus |
nSMase2 | - |
Homo sapiens |
nSMase2 | - |
Rattus norvegicus |
nSMase2 | - |
Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
nSMase2 | Homo sapiens |