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Literature summary for 3.1.4.12 extracted from

  • Ago, H.; Oda, M.; Takahashi, M.; Tsuge, H.; Ochi, S.; Katunuma, N.; Miyano, M.; Sakurai, J.
    Structural basis of the sphingomyelin phosphodiesterase activity in neutral sphingomyelinase from bacillus cereus (2006), J. Biol. Chem., 281, 16157-16167.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, crystal structures of Bc-SMase complexed to the functional metal ions Mg2+, Co2+, or Ca2+ are determined at 1.8 A, 1.8 A, and 1.4 A resolution, respectively Bacillus cereus

Protein Variants

Protein Variants Comment Organism
F285A catalytic activity of the mutant enzyme is below the detection limit, affinity of mutant enzyme to sphingomyelin liposomes are much weaker than the wild-type enzyme Bacillus cereus
W284A catalytic activity of the mutant enzyme is below the detection limit, affinity of mutant enzyme to sphingomyelin liposomes are much weaker than the wild-type enzyme Bacillus cereus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ Km: 0.0191 mM, activation of sphingomyelinase activity in the order of decreasing efficiency Co2+, Mn2+, Mg2+, Ca2+, Sr2+ Bacillus cereus
Co2+ Km: 0.142 mM, activation of sphingomyelinase activity in the order of decreasing efficiency Co2+, Mn2+, Mg2+, Ca2+, Sr2+. There are three distinct metal ion-binding sites in a long horizontal cleft across the bc-SMase molecule Bacillus cereus
Mg2+ Km: 0.0161 mM, activation of sphingomyelinase activity in the order of decreasing efficiency Co2+, Mn2+, Mg2+, Ca2+, Sr2+ Bacillus cereus
Mn2+ Km: 0.0149 mM, activation of sphingomyelinase activity in the order of decreasing efficiency Co2+, Mn2+, Mg2+, Ca2+, Sr2+ Bacillus cereus
Sr2+ Km: 0.018 mM, activation of sphingomyelinase activity in the order of decreasing efficiency Co2+, Mn2+, Mg2+, Ca2+, Sr2+ Bacillus cereus

Organism

Organism UniProt Comment Textmining
Bacillus cereus P11889
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sphingomyelin + H2O
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Bacillus cereus N-acylsphingosine + choline phosphate
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?

Synonyms

Synonyms Comment Organism
Bc-SMase
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Bacillus cereus
neutral sphingomyelinase
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Bacillus cereus