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Literature summary for 3.1.4.12 extracted from

  • Nagahama, M.; Otsuka, A.; Sakurai, J.
    Role of tyrosine-57 and -65 in membrane-damaging and sphingomyelinase activities of Clostridium perfringens alpha-toxin (2006), Biochim. Biophys. Acta, 1762, 110-114.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
H148G mutant enzyme shows no sphingomyelinase activity Clostridium perfringens
Y57A mutant enzyme shows 111.3% of wild-type sphingomyelinase activity Clostridium perfringens
Y57C/C169L mutant enzyme shows 104.1% of wild-type sphingomyelinase activity Clostridium perfringens
Y57F mutant enzyme shows 105.2% of wild-type sphingomyelinase activity Clostridium perfringens
Y57L mutant enzyme shows 103.1% of wild-type sphingomyelinase activity Clostridium perfringens
Y65A mutant enzyme shows 89.4% of wild-type sphingomyelinase activity Clostridium perfringens
Y65C/C169L mutant enzyme shows 98.1% of wild-type sphingomyelinase activity Clostridium perfringens
Y65F mutant enzyme shows 95.6% of wild-type sphingomyelinase activity Clostridium perfringens
Y65L mutant enzyme shows 99.2% of wild-type sphingomyelinase activity Clostridium perfringens

Organism

Organism UniProt Comment Textmining
Clostridium perfringens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
wild-type and mutant enzymes Clostridium perfringens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sphingomyelin + H2O
-
Clostridium perfringens N-acylsphingosine + choline phosphate
-
?

Synonyms

Synonyms Comment Organism
alpha-toxin
-
Clostridium perfringens