Activating Compound | Comment | Organism | Structure |
---|---|---|---|
bile salt | dependent on, particularly taurocholate and taurochenodeoxycholate | Homo sapiens | |
bile salt | dependent on, particularly taurocholate and taurochenodeoxycholate | Rattus norvegicus | |
taurochenodeoxycholate | - |
Homo sapiens | |
taurochenodeoxycholate | - |
Rattus norvegicus | |
taurochenodeoxycholate | stimulates hydrolysis of sphingomyelin and platelet-activating factor | Rattus norvegicus | |
taurocholate | - |
Homo sapiens | |
taurocholate | - |
Rattus norvegicus | |
taurocholate | stimulates hydrolysis of sphingomyelin and platelet-activating factor | Rattus norvegicus |
Cloned (Comment) | Organism |
---|---|
alk-SMase, expression of wild-type and mutant alk-Smase in COS-7 cells | Homo sapiens |
expression in COS-7 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D199A | mutation abolishes hydrolysis of platelet-activating factor | Rattus norvegicus |
D199A | mutation of the metal binding site of alk-SMase abolishes the enzyme activity | Homo sapiens |
D246A | mutation abolishes hydrolysis of platelet-activating factor | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CHAPS | hydrolysis of sphingomyelin and platelet-activating factor | Rattus norvegicus | |
EDTA | alk-SMase activity with platelet-inactivating factor | Homo sapiens | |
EDTA | alk-SMase activity with platelet-inactivating factor; inhibits hydrolysis of platelet-activating factor, no effect on hydrolysis of sphingomyelin | Rattus norvegicus | |
platelet-activating factor | hydrolysis of sphingomyelin | Rattus norvegicus | |
platelet-inactivating factor | competitive inhibition of sphingomyelin hydrolysis | Homo sapiens | |
platelet-inactivating factor | competitive inhibition of sphingomyelin hydrolysis | Rattus norvegicus | |
sphingomyelin | strong competitive inhibition of platelet-inactivating factor hydrolysis | Homo sapiens | |
sphingomyelin | hydrolysis of platelet-activating factor; strong competitive inhibition of platelet-inactivating factor hydrolysis | Rattus norvegicus | |
Triton X-100 | hydrolysis of sphingomyelin and platelet-activating factor | Rattus norvegicus | |
Zn2+ | 0.1-0.25 mM inhibits hydrolysis of platelet-activating factor, concentrations above 0.25 mM inhibit hydrolysis of sphingomyelin and platelet-activating factor | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0136 | - |
platelet-activating factor | - |
Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | 0.1-0.25 mM stimulates hydrolysis of platelet-activating factor | Rattus norvegicus | |
Zn2+ | activates the alk-SMase activity with platelet-inactivating factor, mutation of the metal binding site abolishes the enzyme activity | Homo sapiens | |
Zn2+ | activates the alk-SMase activity with platelet-inactivating factor, mutation of the metal binding site of alk-SMase abolishes the enzyme activity | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
platelet-activating factor + H2O | Homo sapiens | the enzyme cleaves the phosphocholine head group from platelet-activating factor. Potential protective effect of the enzyme against inflammatory bowel disease and colon cancer | 1-O-alkyl-2-acetyl-sn-glycerol + choline phosphate | - |
? | |
platelet-activating factor + H2O | Rattus norvegicus | the enzyme cleaves the phosphocholine head group from platelet-activating factor. Potential protective effect of the enzyme against inflammatory bowel disease and colon cancer | 1-O-alkyl-2-acetyl-sn-glycerol + choline phosphate | - |
? | |
platelet-inactivating factor + H2O | Homo sapiens | alk-SMase hydrolyses and inactivates platelet-activating factor by a phospholipase C activity, alk-SMase cleaves the phosphocholine head group from PAF and generates 1-O-alkyl-2-acetyl-sn-glycerol | 1-O-alkyl-2-acetyl-sn-glycerol + choline phosphate + ? | - |
? | |
platelet-inactivating factor + H2O | Rattus norvegicus | alk-SMase hydrolyses and inactivates platelet-activating factor by a phospholipase C activity, alk-SMase cleaves the phosphocholine head group from PAF and generates 1-O-alkyl-2-acetyl-sn-glycerol | 1-O-alkyl-2-acetyl-sn-glycerol + choline phosphate + ? | - |
? | |
sphingomyelin + H2O | Rattus norvegicus | intestinal alkaline sphingomyelinase hydrolyses sphingomyelin to generate ceramide in the intestinal tract, and hydrolyses and inactivates platelet-activating factor by a phospholipase C activity, it may protect the intestinal mucosa from inflammation and tumorigenesis, overview | N-acylsphingosine + choline phosphate | - |
? | |
sphingomyelin + H2O | Homo sapiens | intestinal alkaline sphingomyelinase hydrolyses sphingomyelin to generate ceramide in the intestinal tract, it may protect the intestinal mucosa from inflammation and tumorigenesis, overview | N-acylsphingosine + choline phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant alk-SMase from COS-7 cells | Rattus norvegicus |
recombinant wild-type and mutant alk-Smase from COS-7 cells | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HT-29 cell | - |
Homo sapiens | - |
intestine | - |
Homo sapiens | - |
intestine | - |
Rattus norvegicus | - |
leukocyte | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Homo sapiens |
additional information | - |
- |
Rattus norvegicus |
6.23 | - |
purified enzyme with substrate platelet-inactivating factor | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
platelet-activating factor + H2O | the enzyme cleaves the phosphocholine head group from platelet-activating factor. Potential protective effect of the enzyme against inflammatory bowel disease and colon cancer | Homo sapiens | 1-O-alkyl-2-acetyl-sn-glycerol + choline phosphate | - |
? | |
platelet-activating factor + H2O | the enzyme cleaves the phosphocholine head group from platelet-activating factor. Potential protective effect of the enzyme against inflammatory bowel disease and colon cancer | Rattus norvegicus | 1-O-alkyl-2-acetyl-sn-glycerol + choline phosphate | - |
? | |
platelet-activating factor + H2O | the enzyme cleaves the phosphocholine head group from platelet-activating factor | Homo sapiens | 1-O-alkyl-2-acetyl-sn-glycerol + choline phosphate | - |
? | |
platelet-activating factor + H2O | the enzyme cleaves the phosphocholine head group from platelet-activating factor | Rattus norvegicus | 1-O-alkyl-2-acetyl-sn-glycerol + choline phosphate | - |
? | |
platelet-inactivating factor + H2O | - |
Homo sapiens | 1-O-alkyl-2-acetyl-sn-glycerol + choline phosphate + ? | - |
? | |
platelet-inactivating factor + H2O | - |
Rattus norvegicus | 1-O-alkyl-2-acetyl-sn-glycerol + choline phosphate + ? | - |
? | |
platelet-inactivating factor + H2O | alk-SMase hydrolyses and inactivates platelet-activating factor by a phospholipase C activity, alk-SMase cleaves the phosphocholine head group from PAF and generates 1-O-alkyl-2-acetyl-sn-glycerol | Homo sapiens | 1-O-alkyl-2-acetyl-sn-glycerol + choline phosphate + ? | - |
? | |
platelet-inactivating factor + H2O | alk-SMase hydrolyses and inactivates platelet-activating factor by a phospholipase C activity, alk-SMase cleaves the phosphocholine head group from PAF and generates 1-O-alkyl-2-acetyl-sn-glycerol | Rattus norvegicus | 1-O-alkyl-2-acetyl-sn-glycerol + choline phosphate + ? | - |
? | |
sphingomyelin + H2O | - |
Homo sapiens | N-acylsphingosine + choline phosphate | - |
? | |
sphingomyelin + H2O | - |
Rattus norvegicus | N-acylsphingosine + choline phosphate | - |
? | |
sphingomyelin + H2O | intestinal alkaline sphingomyelinase hydrolyses sphingomyelin to generate ceramide in the intestinal tract, and hydrolyses and inactivates platelet-activating factor by a phospholipase C activity, it may protect the intestinal mucosa from inflammation and tumorigenesis, overview | Rattus norvegicus | N-acylsphingosine + choline phosphate | - |
? | |
sphingomyelin + H2O | intestinal alkaline sphingomyelinase hydrolyses sphingomyelin to generate ceramide in the intestinal tract, it may protect the intestinal mucosa from inflammation and tumorigenesis, overview | Homo sapiens | N-acylsphingosine + choline phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
alk-SMase | - |
Homo sapiens |
alk-SMase | - |
Rattus norvegicus |
alkaline sphingomyelinase | - |
Homo sapiens |
alkaline sphingomyelinase | - |
Rattus norvegicus |
intestinal alkaline sphingomyelinase | - |
Homo sapiens |
intestinal alkaline sphingomyelinase | - |
Rattus norvegicus |
More | alk-SMase is a member of the NPP, i.e. nucleotide diphosphatase/phosphodiesterase, family | Homo sapiens |
More | alk-SMase is a member of the NPP, i.e. nucleotide diphosphatase/phosphodiesterase, family | Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
37 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
hydrolysis of platelet-activating factor | Rattus norvegicus |
7.5 | - |
alk-SMase activity with platelet-inactivating factor | Homo sapiens |
7.5 | - |
alk-SMase activity with platelet-inactivating factor | Rattus norvegicus |
9 | - |
hydrolysis of sphingomyelin | Rattus norvegicus |
9 | - |
alk-SMase activity on sphingomyelin | Homo sapiens |
9 | - |
alk-SMase activity on sphingomyelin | Rattus norvegicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 10 | - |
Homo sapiens |
5 | 10 | - |
Rattus norvegicus |
6 | 9 | pH 6.0: about 55% of maximal activity, pH 9.0: about 35% of maximal activity, hydrolysis of platelet-activating factor | Rattus norvegicus |