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Literature summary for 3.1.4.12 extracted from

  • Hofmann, K.; Tomiuk, S.; Wolff, G.; Stoffel, W.
    Cloning and characterization of the mammalian brain-specific, Mg2+-dependent neutral sphingomyelinase (2000), Proc. Natl. Acad. Sci. USA, 97, 5895-5900.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
dioleoyl-phosphatidylserine 0.05 mM, 4fold activation Homo sapiens
phosphatidylethanol 0.05 mM, 2fold activation Homo sapiens
phosphatidylserine 0.05 mM, 4fold activation Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
cloning of cDNA Homo sapiens
cloning of cDNA, expression in HEK293 kidney cells Mus musculus

General Stability

General Stability Organism
reducing agents such as dithiothreitol stablize nSMase2 during preparation Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
EDTA 5 mM, complete inhibition, activity is comletely restored by the subsequent addition of 10 mM Mg2+ or Mn2+ Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Homo sapiens 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required for activity Homo sapiens
Mn2+ can replace Mn2+ Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
71000
-
x * 71000, deduced from nucleotide sequence Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sphingomyelin + H2O Homo sapiens
-
N-acylsphingosine + choline phosphate
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9NY59
-
-

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
Homo sapiens
-
liver
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sphingomyelin + H2O
-
Homo sapiens N-acylsphingosine + choline phosphate
-
?

Subunits

Subunits Comment Organism
? x * 71000, deduced from nucleotide sequence Homo sapiens

Synonyms

Synonyms Comment Organism
neutral sphingomyelinase 2
-
Homo sapiens
neutral sphingomylinase 2
-
Homo sapiens
nSMase2
-
Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
4.5 9 approx. 5% of maximal activity at pH 4.5, approx. 15% of maximal activity at pH 9.0 Homo sapiens