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Literature summary for 3.1.4.12 extracted from

  • Lansmann, S.; Bartelsen, O.; Sandhoff, K.
    Purification and characterization of recombinant human acid sphingomyelinase expressed in insect Sf21 cells (2000), Methods Enzymol., 311, 149-156.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Sf9 insect cells Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.032
-
sphingomyelin 37°C, pH 4.8 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
lysosome
-
Homo sapiens 5764
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
72000
-
native PAGE, recombinant haSMase Homo sapiens
75000
-
native PAGE, placental haSMase Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sphingomyelin + H2O Homo sapiens
-
N-acylsphingosine + choline phosphate
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein molecular mass reduction of approx. 8-9 kDA upon deglycosylation Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
recombinant haSMase, ammonium sulfate, Con A-Sepharose, Octyl-Sepharose, Matrex Gel Red A, DEAE-650 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
placenta
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.33
-
-
Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sphingomyelin + H2O
-
Homo sapiens N-acylsphingosine + choline phosphate
-
?

Synonyms

Synonyms Comment Organism
acid sphingomyelinase
-
Homo sapiens
haSMase
-
Homo sapiens