Activating Compound | Comment | Organism | Structure |
---|---|---|---|
sodium deoxycholate | 4.4fold activation of S-mSMase epsilon at 2 mM, 4.7fold activation of S-mSMase zeta at 1 mM | Bos taurus | |
Triton X-100 | 0.02%, approx. 3fold activation of T-mSMase gamma | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
GSH | inhibition of all T-mSMase and S-mSMase isoforms above 2.5 mM | Bos taurus | |
Triton X-100 | - |
Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | associated | Bos taurus | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | approx. 2fold activation of T-mSMase isoforms delta and gamma at 1 mM and 5 mM, respectively, both S-mSMase isoforms epsilon and zeta require 5 mM Mg2+ for maximal activity | Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
x * 30000, SDS-PAGE, T-mSMase isoforms alpha, beta, gamma and delta | Bos taurus |
600000 | - |
gel filtration, T-mSMase isoforms alpha, beta, gamma and delta | Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sphingomyelin + H2O | Bos taurus | - |
N-acylsphingosine + choline phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Triton X-100, DE52, Butyl-Toyopearl, DEAE-5PW, Sephacryl S-300, Mono S, T-mSMase isoforms alpha, beta, gamma and delta, ammonium sulfate, Butyl-Toyopearl, Sephacryl S-300, Phenyl-5PW, DEAE-5PW, Resource S, S-mSMase isoforms epsilon and zeta | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.021 | - |
S-mSMase zeta | Bos taurus |
0.024 | - |
S-mSMase epsilon | Bos taurus |
0.033 | - |
T-mSMase gamma | Bos taurus |
0.051 | - |
T-mSMase delta | Bos taurus |
0.063 | - |
T-mSMase alpha | Bos taurus |
0.11 | - |
T-mSMase beta | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sphingomyelin + H2O | - |
Bos taurus | N-acylsphingosine + choline phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 30000, SDS-PAGE, T-mSMase isoforms alpha, beta, gamma and delta | Bos taurus |
Synonyms | Comment | Organism |
---|---|---|
membrane associated neutral sphingomyelinase | - |
Bos taurus |
N-mSMase | - |
Bos taurus |
S-mSMase | ammonium sulfate extracted isoforms epsilon and zeta | Bos taurus |
T-mSMase | Triton X-100 isoforms alpha, beta, gamma and delta | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
T-mSMase isoform alpha | Bos taurus |
7 | - |
T-mSMase isoforms beta and gamma, S-mSMase zeta | Bos taurus |
7.5 | - |
T-mSMase isoforms alpha and delta | Bos taurus |
7.5 | 8 | S-mSMase isoform epsilon | Bos taurus |
8 | - |
S-mSMase isoform zeta | Bos taurus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 10 | T-mSMase alpha, approx. 50% of maximal activity at pH 4.5, T-mSMase isoforms beta, gamma and delta, approx. 25% of maximal activity at pH 4.5, T-mSMase isoforms alpha, beta, gamma and delta, almost no activity at pH 10.0 | Bos taurus |