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Literature summary for 3.1.4.12 extracted from

  • Jung, S.Y.; Suh, J.H.; Park, H.J.; Jung, K.M.; Kim, M.Y.; Na, D.S.; Kim, D.K.
    Identification of multiple forms of membrane-associated neutral sphingomyelinase in bovine brain (2000), J. Neurochem., 75, 1004-1014.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
sodium deoxycholate 4.4fold activation of S-mSMase epsilon at 2 mM, 4.7fold activation of S-mSMase zeta at 1 mM Bos taurus
Triton X-100 0.02%, approx. 3fold activation of T-mSMase gamma Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
GSH inhibition of all T-mSMase and S-mSMase isoforms above 2.5 mM Bos taurus
Triton X-100
-
Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane associated Bos taurus 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ approx. 2fold activation of T-mSMase isoforms delta and gamma at 1 mM and 5 mM, respectively, both S-mSMase isoforms epsilon and zeta require 5 mM Mg2+ for maximal activity Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
x * 30000, SDS-PAGE, T-mSMase isoforms alpha, beta, gamma and delta Bos taurus
600000
-
gel filtration, T-mSMase isoforms alpha, beta, gamma and delta Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sphingomyelin + H2O Bos taurus
-
N-acylsphingosine + choline phosphate
-
?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Triton X-100, DE52, Butyl-Toyopearl, DEAE-5PW, Sephacryl S-300, Mono S, T-mSMase isoforms alpha, beta, gamma and delta, ammonium sulfate, Butyl-Toyopearl, Sephacryl S-300, Phenyl-5PW, DEAE-5PW, Resource S, S-mSMase isoforms epsilon and zeta Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.021
-
S-mSMase zeta Bos taurus
0.024
-
S-mSMase epsilon Bos taurus
0.033
-
T-mSMase gamma Bos taurus
0.051
-
T-mSMase delta Bos taurus
0.063
-
T-mSMase alpha Bos taurus
0.11
-
T-mSMase beta Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sphingomyelin + H2O
-
Bos taurus N-acylsphingosine + choline phosphate
-
?

Subunits

Subunits Comment Organism
? x * 30000, SDS-PAGE, T-mSMase isoforms alpha, beta, gamma and delta Bos taurus

Synonyms

Synonyms Comment Organism
membrane associated neutral sphingomyelinase
-
Bos taurus
N-mSMase
-
Bos taurus
S-mSMase ammonium sulfate extracted isoforms epsilon and zeta Bos taurus
T-mSMase Triton X-100 isoforms alpha, beta, gamma and delta Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
T-mSMase isoform alpha Bos taurus
7
-
T-mSMase isoforms beta and gamma, S-mSMase zeta Bos taurus
7.5
-
T-mSMase isoforms alpha and delta Bos taurus
7.5 8 S-mSMase isoform epsilon Bos taurus
8
-
S-mSMase isoform zeta Bos taurus

pH Range

pH Minimum pH Maximum Comment Organism
4.5 10 T-mSMase alpha, approx. 50% of maximal activity at pH 4.5, T-mSMase isoforms beta, gamma and delta, approx. 25% of maximal activity at pH 4.5, T-mSMase isoforms alpha, beta, gamma and delta, almost no activity at pH 10.0 Bos taurus