Activating Compound | Comment | Organism | Structure |
---|---|---|---|
taurochenodeoxycholate | strong activation, maximal activation at approx. 2 mM | Rattus norvegicus | |
taurocholate | strong activation, maximal activation at approx. 10 mM | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | 10 mM, 25% inhibition | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.25 | - |
sphingomyelin | 37°C, pH 9.0 | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58000 | - |
x * 58000, SDS-PAGE | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sphingomyelin + H2O | Rattus norvegicus | - |
N-acylsphingosine + choline phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
acetone, DEAE-Sepharose, High Q, Phenyl-Sepharose, SE-column | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
intestine | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
14.8 | - |
- |
Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphatidylcholine + H2O | 8% of activity with sphingomyelin | Rattus norvegicus | diacylglycerol + choline phosphate | - |
? | |
sphingomyelin + H2O | - |
Rattus norvegicus | N-acylsphingosine + choline phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 58000, SDS-PAGE | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
alkaline SMase | - |
Rattus norvegicus |
alkaline sphingomyelinase | - |
Rattus norvegicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
no loss of activity after 1 h | Rattus norvegicus |
60 | - |
complete loss of activity | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | 9.5 | - |
Rattus norvegicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 10 | almost no activity at pH 6.0, approx. 60% of maximal activity at pH 7.5, approx. 90% of maximal activity at Ph 10.0 | Rattus norvegicus |