Activating Compound | Comment | Organism | Structure |
---|---|---|---|
cardiolipin | 0.1 mM, approx. 40fold activation | Mus musculus | |
phosphatidylserine | 0.1-0.15 mM, approx. 40fold activation | Mus musculus | |
Triton X-100 | required for activity, maximal activation at 0.1% | Mus musculus |
Cloned (Comment) | Organism |
---|---|
expression of nSMase2 in Saccharomyces cerevisiae and MCF7 breast cancer cells | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
GW4869 | 0.015 mM, 40% inhibition, 0.045 mM, 70% inhibition | Mus musculus | |
Mn2+ | slight inhibition above 2 mM | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.027 | - |
sphingomyelin | 37°C, pH 7.4, recombinant nSMase2 in yeast microsomes | Mus musculus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required for activity, maximal activation at 5 mM | Mus musculus | |
Mn2+ | can replace Mg2+, maximal activation at 1-2 mM, slight inhibition above | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sphingomyelin + H2O | Mus musculus | - |
N-acylsphingosine + choline phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | Q9JJY3 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sphingomyelin + H2O | - |
Mus musculus | N-acylsphingosine + choline phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
neutral sphingomyelinase | - |
Mus musculus |
nSMase2 | - |
Mus musculus |