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Literature summary for 3.1.4.12 extracted from

  • Bernardo, K.; Krut, O.; Wiegmann, K.; Kreder, D.; Micheli, M.; Schafer, R.; Sickman, A.; Schmidt, W.E.; Schroder, J.M.; Meyer, H.E.; Sandhoff, K.; Kronke, M.
    Purification and characterization of a magnesium-dependent neutral sphingomyelinase from bovine brain (2000), J. Biol. Chem., 275, 7641-7647.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
phosphatidylcholine 0.2 mM, 2fold activation Bos taurus
phosphatidylethanolamine 0.2 mM, 2.9fold activation Bos taurus
phosphatidylserine 0.2 mM, 3.8fold activation Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 0.1 mM, 60% inhibition of Mg2+-activated N-SMase Bos taurus
EDTA 10 mM, 95% inhibition Bos taurus
glutathione 0.2 mM, almost complete inhibition Bos taurus
scyphostatin 0.02 mM, complete inhibition Bos taurus
Zn2+ almost complete inhibition of Mg2+-activated N-SMase Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
sphingomyelin 37°C, pH 7.4 Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
plasma membrane associated Bos taurus 5886
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required for activity, approx. 3fold activation at 0.1-0.5 mM Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
97000
-
x * 97000, 2D-electrophoresis Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sphingomyelin + H2O Bos taurus
-
N-acylsphingosine + choline phosphate
-
?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
NP-40, CM-Sepharose, concanavalin A-Sepharose, Methyl-macroprep, isoelectric focusing Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.23
-
-
Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sphingomyelin + H2O
-
Bos taurus N-acylsphingosine + choline phosphate
-
?

Subunits

Subunits Comment Organism
? x * 97000, 2D-electrophoresis Bos taurus

Synonyms

Synonyms Comment Organism
N-SMase
-
Bos taurus
neutral sphingomyelinase
-
Bos taurus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
56
-
95% loss of activity after 5 min Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8
-
Bos taurus

pH Range

pH Minimum pH Maximum Comment Organism
6 9 approx. 30% of maximal activity at pH 6.5, approx. 70% of maximal activity at pH 9.0 Bos taurus

pI Value

Organism Comment pI Value Maximum pI Value
Bos taurus isoelectric focusing
-
5.3