Activating Compound | Comment | Organism | Structure |
---|---|---|---|
phosphatidylcholine | 0.2 mM, 2fold activation | Bos taurus | |
phosphatidylethanolamine | 0.2 mM, 2.9fold activation | Bos taurus | |
phosphatidylserine | 0.2 mM, 3.8fold activation | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 0.1 mM, 60% inhibition of Mg2+-activated N-SMase | Bos taurus | |
EDTA | 10 mM, 95% inhibition | Bos taurus | |
glutathione | 0.2 mM, almost complete inhibition | Bos taurus | |
scyphostatin | 0.02 mM, complete inhibition | Bos taurus | |
Zn2+ | almost complete inhibition of Mg2+-activated N-SMase | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
sphingomyelin | 37°C, pH 7.4 | Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
plasma membrane | associated | Bos taurus | 5886 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required for activity, approx. 3fold activation at 0.1-0.5 mM | Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
97000 | - |
x * 97000, 2D-electrophoresis | Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sphingomyelin + H2O | Bos taurus | - |
N-acylsphingosine + choline phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
NP-40, CM-Sepharose, concanavalin A-Sepharose, Methyl-macroprep, isoelectric focusing | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.23 | - |
- |
Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sphingomyelin + H2O | - |
Bos taurus | N-acylsphingosine + choline phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 97000, 2D-electrophoresis | Bos taurus |
Synonyms | Comment | Organism |
---|---|---|
N-SMase | - |
Bos taurus |
neutral sphingomyelinase | - |
Bos taurus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
56 | - |
95% loss of activity after 5 min | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | - |
Bos taurus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | approx. 30% of maximal activity at pH 6.5, approx. 70% of maximal activity at pH 9.0 | Bos taurus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Bos taurus | isoelectric focusing | - |
5.3 |