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Literature summary for 3.1.4.12 extracted from

  • Tomiuk, S.; Zumbansen, M.; Stoffel, W.
    Characterization and subcellular localization of murine and human magnesium-dependent neutral sphingomyelinase (2000), J. Biol. Chem., 275, 5710-5717.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
arachidonic acid approx. 3fold activation at 0.5 mM Mus musculus
arachidonic acid approx. 3fold activation at 0.5 mM Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli and HEK293 kidney cells Mus musculus
expression in Escherichia coli and HEK293 kidney cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
E49Q complete loss of hnSMase activity Homo sapiens
H272N complete loss of hnSMase activity Homo sapiens
N180H complete loss of hnSMase activity Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Mn2+ above 0.5 mM Homo sapiens
Mn2+ above 0.5 mM Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
smooth endoplasmic reticulum
-
Mus musculus 5790
-
smooth endoplasmic reticulum
-
Homo sapiens 5790
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required for activity, maximal activation at 2-5 mM Mus musculus
Mg2+ required for activity, maximal activation at 2-5 mM Homo sapiens
Mn2+ can replace Mg2+, maximal activity at 0.5 mM, inhibition above Mus musculus
Mn2+ can replace Mg2+, maximal activity at 0.5 mM, inhibition above Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
x * 42000, SDS-PAGE Mus musculus
42000
-
x * 42000, SDS-PAGE Homo sapiens
47500
-
x * 47500, deduced from nucleotide sequence Mus musculus
47600
-
x * 47600, deduced from nucleotide sequence Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sphingomyelin + H2O Mus musculus
-
N-acylsphingosine + choline phosphate
-
?
sphingomyelin + H2O Homo sapiens
-
N-acylsphingosine + choline phosphate
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Mus musculus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
no glycoprotein
-
Mus musculus
no glycoprotein
-
Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
affinity purification of recombinant hnSMase Homo sapiens
affinity purification of recombinant mnSMase Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
Mus musculus
-
brain
-
Homo sapiens
-
heart
-
Mus musculus
-
heart
-
Homo sapiens
-
kidney predominant tissue Mus musculus
-
kidney predominant tissue Homo sapiens
-
liver
-
Mus musculus
-
liver
-
Homo sapiens
-
lung
-
Mus musculus
-
lung
-
Homo sapiens
-
muscle
-
Mus musculus
-
muscle
-
Homo sapiens
-
spleen
-
Mus musculus
-
spleen
-
Homo sapiens
-
testis
-
Mus musculus
-
testis
-
Homo sapiens
-
thymus
-
Mus musculus
-
thymus
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sphingomyelin + H2O
-
Mus musculus N-acylsphingosine + choline phosphate
-
?
sphingomyelin + H2O
-
Homo sapiens N-acylsphingosine + choline phosphate
-
?

Subunits

Subunits Comment Organism
? x * 42000, SDS-PAGE Mus musculus
? x * 42000, SDS-PAGE Homo sapiens
? x * 47500, deduced from nucleotide sequence Mus musculus
? x * 47600, deduced from nucleotide sequence Homo sapiens

Synonyms

Synonyms Comment Organism
hnSMase
-
Homo sapiens
magnesium-dependent neutral sphingomyelinase
-
Mus musculus
magnesium-dependent neutral sphingomyelinase
-
Homo sapiens
mnSMase
-
Mus musculus