Activating Compound | Comment | Organism | Structure |
---|---|---|---|
arachidonic acid | approx. 3fold activation at 0.5 mM | Mus musculus | |
arachidonic acid | approx. 3fold activation at 0.5 mM | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli and HEK293 kidney cells | Mus musculus |
expression in Escherichia coli and HEK293 kidney cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
E49Q | complete loss of hnSMase activity | Homo sapiens |
H272N | complete loss of hnSMase activity | Homo sapiens |
N180H | complete loss of hnSMase activity | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | above 0.5 mM | Homo sapiens | |
Mn2+ | above 0.5 mM | Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
smooth endoplasmic reticulum | - |
Mus musculus | 5790 | - |
smooth endoplasmic reticulum | - |
Homo sapiens | 5790 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required for activity, maximal activation at 2-5 mM | Mus musculus | |
Mg2+ | required for activity, maximal activation at 2-5 mM | Homo sapiens | |
Mn2+ | can replace Mg2+, maximal activity at 0.5 mM, inhibition above | Mus musculus | |
Mn2+ | can replace Mg2+, maximal activity at 0.5 mM, inhibition above | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
x * 42000, SDS-PAGE | Mus musculus |
42000 | - |
x * 42000, SDS-PAGE | Homo sapiens |
47500 | - |
x * 47500, deduced from nucleotide sequence | Mus musculus |
47600 | - |
x * 47600, deduced from nucleotide sequence | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sphingomyelin + H2O | Mus musculus | - |
N-acylsphingosine + choline phosphate | - |
? | |
sphingomyelin + H2O | Homo sapiens | - |
N-acylsphingosine + choline phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Mus musculus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
no glycoprotein | - |
Mus musculus |
no glycoprotein | - |
Homo sapiens |
Purification (Comment) | Organism |
---|---|
affinity purification of recombinant hnSMase | Homo sapiens |
affinity purification of recombinant mnSMase | Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Mus musculus | - |
brain | - |
Homo sapiens | - |
heart | - |
Mus musculus | - |
heart | - |
Homo sapiens | - |
kidney | predominant tissue | Mus musculus | - |
kidney | predominant tissue | Homo sapiens | - |
liver | - |
Mus musculus | - |
liver | - |
Homo sapiens | - |
lung | - |
Mus musculus | - |
lung | - |
Homo sapiens | - |
muscle | - |
Mus musculus | - |
muscle | - |
Homo sapiens | - |
spleen | - |
Mus musculus | - |
spleen | - |
Homo sapiens | - |
testis | - |
Mus musculus | - |
testis | - |
Homo sapiens | - |
thymus | - |
Mus musculus | - |
thymus | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sphingomyelin + H2O | - |
Mus musculus | N-acylsphingosine + choline phosphate | - |
? | |
sphingomyelin + H2O | - |
Homo sapiens | N-acylsphingosine + choline phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 42000, SDS-PAGE | Mus musculus |
? | x * 42000, SDS-PAGE | Homo sapiens |
? | x * 47500, deduced from nucleotide sequence | Mus musculus |
? | x * 47600, deduced from nucleotide sequence | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
hnSMase | - |
Homo sapiens |
magnesium-dependent neutral sphingomyelinase | - |
Mus musculus |
magnesium-dependent neutral sphingomyelinase | - |
Homo sapiens |
mnSMase | - |
Mus musculus |