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Literature summary for 3.1.4.12 extracted from

  • Obama, T.; Kan, Y.; Ikezawa, H.; Imagawa, M.; Tsukamoto, K.
    Glu-53 of Bacillus cereus sphingomyelinase acts as an indispensable ligand of Mg2+ essential for catalytic activity (2003), J. Biochem., 133, 279-286.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Bacillus brevis Bacillus cereus

Protein Variants

Protein Variants Comment Organism
E53A no sphingomyelin hydrolyzing and no hemolytic activity, E53 acts as an indespensable ligand of Mg2+ essential for catalytic activity Bacillus cereus
E53D 5% of wild-type sphingomyelin hydrolyzing activity, E53 acts as an indespensable ligand of Mg2+ essential for catalytic activity Bacillus cereus
E53Q no sphingomyelin hydrolyzing and no hemolytic activity, E53 acts as an indespensable ligand of Mg2+ essential for catalytic activity Bacillus cereus

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 6.9 mM, 50% inhibitiion Bacillus cereus
Cu2+ 0.0079 mM, 50% inhibitiion Bacillus cereus
Zn2+ 0.0042 mM, 50% inhibitiion, higher concentrations of Mg2+ slighty restore activity Bacillus cereus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.35
-
sphingomyelin 37°C, pH 7.5 Bacillus cereus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Bacillus cereus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ can replace Mg2+, 50% activation at 0.0015 mM Bacillus cereus
Mg2+ two-step activation, high-affinity binding site with 50% saturation at 0.0041 mM, low-affinity-binding site with 50% saturation at 20 mM, approx. 5fold activation at 20 mM Bacillus cereus
Mn2+ can replace Mg2+, 50% activation at 0.00087 mM Bacillus cereus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sphingomyelin + H2O Bacillus cereus
-
N-acylsphingosine + choline phosphate
-
?

Organism

Organism UniProt Comment Textmining
Bacillus cereus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant SMase Bacillus cereus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-hexadecanoylamino-4-nitrophenylphosphocholine + H2O
-
Bacillus cereus 2-hexadecanoylamino-4-nitrophenol + choline phosphate
-
?
lysophosphatidylcholine + H2O
-
Bacillus cereus acylglycerol + choline phosphate
-
?
sphingomyelin + H2O
-
Bacillus cereus N-acylsphingosine + choline phosphate
-
?

Synonyms

Synonyms Comment Organism
SMase
-
Bacillus cereus
sphingomyelinase
-
Bacillus cereus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.9
-
2-hexadecanoylamino-4-nitrophenylphosphocholine 37°C, pH 7.5 Bacillus cereus
13
-
lysophosphatidylcholine 37°C, pH 7.5 Bacillus cereus
210
-
sphingomyelin 37°C, pH 7.5 Bacillus cereus