Cloned (Comment) | Organism |
---|---|
expression in Bacillus brevis | Bacillus cereus |
Protein Variants | Comment | Organism |
---|---|---|
E53A | no sphingomyelin hydrolyzing and no hemolytic activity, E53 acts as an indespensable ligand of Mg2+ essential for catalytic activity | Bacillus cereus |
E53D | 5% of wild-type sphingomyelin hydrolyzing activity, E53 acts as an indespensable ligand of Mg2+ essential for catalytic activity | Bacillus cereus |
E53Q | no sphingomyelin hydrolyzing and no hemolytic activity, E53 acts as an indespensable ligand of Mg2+ essential for catalytic activity | Bacillus cereus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 6.9 mM, 50% inhibitiion | Bacillus cereus | |
Cu2+ | 0.0079 mM, 50% inhibitiion | Bacillus cereus | |
Zn2+ | 0.0042 mM, 50% inhibitiion, higher concentrations of Mg2+ slighty restore activity | Bacillus cereus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.35 | - |
sphingomyelin | 37°C, pH 7.5 | Bacillus cereus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Bacillus cereus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | can replace Mg2+, 50% activation at 0.0015 mM | Bacillus cereus | |
Mg2+ | two-step activation, high-affinity binding site with 50% saturation at 0.0041 mM, low-affinity-binding site with 50% saturation at 20 mM, approx. 5fold activation at 20 mM | Bacillus cereus | |
Mn2+ | can replace Mg2+, 50% activation at 0.00087 mM | Bacillus cereus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sphingomyelin + H2O | Bacillus cereus | - |
N-acylsphingosine + choline phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus cereus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant SMase | Bacillus cereus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-hexadecanoylamino-4-nitrophenylphosphocholine + H2O | - |
Bacillus cereus | 2-hexadecanoylamino-4-nitrophenol + choline phosphate | - |
? | |
lysophosphatidylcholine + H2O | - |
Bacillus cereus | acylglycerol + choline phosphate | - |
? | |
sphingomyelin + H2O | - |
Bacillus cereus | N-acylsphingosine + choline phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SMase | - |
Bacillus cereus |
sphingomyelinase | - |
Bacillus cereus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.9 | - |
2-hexadecanoylamino-4-nitrophenylphosphocholine | 37°C, pH 7.5 | Bacillus cereus | |
13 | - |
lysophosphatidylcholine | 37°C, pH 7.5 | Bacillus cereus | |
210 | - |
sphingomyelin | 37°C, pH 7.5 | Bacillus cereus |