Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.4.12 extracted from

  • Sueyoshi, N.; Kita, K.; Okino, N.; Sakaguchi, K.; Nakamura, T.; Ito, M.
    Molecular cloning and expression of Mn2+-dependent sphingomyelinase/hemolysin of an aquatic bacterium, Pseudomonas sp. strain TK4 (2002), J. Bacteriol., 184, 540-546.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pseudomonas sp.

Protein Variants

Protein Variants Comment Organism
additional information C-Terminal deletion mutants lose sphingomyelinase activity for hydrolysis of intact erythrocytes Pseudomonas sp.

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ strong inhibition of hemolysis at 10 mM Pseudomonas sp.
Cu2+ 5 mM, complete inhibition Pseudomonas sp.
EDTA 5 mM, complete inhibition, subsequent addition of Mn2+, Mg2+ or Ca2+ restores activity to 300% of that before EDTA treatment Pseudomonas sp.
Hg2+ 5 mM, complete inhibition Pseudomonas sp.
Ni2+ 5 mM, complete inhibition Pseudomonas sp.
Zn2+ 5 mM, complete inhibition Pseudomonas sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activation of hemolysis at 2 mM, strong inhibition at 10 mM Pseudomonas sp.
Mg2+ 5 mM, activation Pseudomonas sp.
Mn2+ 5 mM, activation Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
58110
-
x * 58110, deduced from nucleotide sequence Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sphingomyelin + H2O Pseudomonas sp. SMase C causes hemolysis and thus is suspected to be a potential virulence factor N-acylsphingosine + choline phosphate
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
HighTrap Phenyl-sepharose, HiTrap Q, recombinant SMase Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
33
-
recombinant SMase C Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrobenz-2-oxa-1,3-diazole-sphingosylphosphorylcholine + H2O
-
Pseudomonas sp. 4-nitrobenz-2-oxa-1,3-diazole-sphingosine + choline phosphate
-
?
lysophosphatidylcholine + H2O 8% of activity with sphingomyelin Pseudomonas sp. acylglycerol + choline phosphate
-
?
N-dodecanoyl-4-nitrobenz-2-oxa-1,3-diazole-sphingomyelin + H2O
-
Pseudomonas sp. 4-nitrobenz-2-oxa-1,3-diazole-N-dodecanoylsphingosine + choline phosphate
-
?
N-hexanoyl-4-nitrobenz-2-oxa-1,3-diazole-sphingomyelin + H2O
-
Pseudomonas sp. 4-nitrobenz-2-oxa-1,3-diazole-N-hexanoylsphingosine + choline phosphate
-
?
sphingomyelin + H2O
-
Pseudomonas sp. N-acylsphingosine + choline phosphate
-
?
sphingomyelin + H2O SMase C causes hemolysis and thus is suspected to be a potential virulence factor Pseudomonas sp. N-acylsphingosine + choline phosphate
-
?
sphingosylphosphorylcholine + H2O 25% of activity with sphingomyelin Pseudomonas sp. sphingosine + choline phosphate
-
?

Subunits

Subunits Comment Organism
? x * 58110, deduced from nucleotide sequence Pseudomonas sp.

Synonyms

Synonyms Comment Organism
SMase C
-
Pseudomonas sp.
sphingomyelinase/hemolysin
-
Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Pseudomonas sp.

pI Value

Organism Comment pI Value Maximum pI Value
Pseudomonas sp. deduced from nucleotide sequence
-
6.33