Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pseudomonas sp. |
Protein Variants | Comment | Organism |
---|---|---|
additional information | C-Terminal deletion mutants lose sphingomyelinase activity for hydrolysis of intact erythrocytes | Pseudomonas sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | strong inhibition of hemolysis at 10 mM | Pseudomonas sp. | |
Cu2+ | 5 mM, complete inhibition | Pseudomonas sp. | |
EDTA | 5 mM, complete inhibition, subsequent addition of Mn2+, Mg2+ or Ca2+ restores activity to 300% of that before EDTA treatment | Pseudomonas sp. | |
Hg2+ | 5 mM, complete inhibition | Pseudomonas sp. | |
Ni2+ | 5 mM, complete inhibition | Pseudomonas sp. | |
Zn2+ | 5 mM, complete inhibition | Pseudomonas sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activation of hemolysis at 2 mM, strong inhibition at 10 mM | Pseudomonas sp. | |
Mg2+ | 5 mM, activation | Pseudomonas sp. | |
Mn2+ | 5 mM, activation | Pseudomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58110 | - |
x * 58110, deduced from nucleotide sequence | Pseudomonas sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sphingomyelin + H2O | Pseudomonas sp. | SMase C causes hemolysis and thus is suspected to be a potential virulence factor | N-acylsphingosine + choline phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
HighTrap Phenyl-sepharose, HiTrap Q, recombinant SMase | Pseudomonas sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
33 | - |
recombinant SMase C | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrobenz-2-oxa-1,3-diazole-sphingosylphosphorylcholine + H2O | - |
Pseudomonas sp. | 4-nitrobenz-2-oxa-1,3-diazole-sphingosine + choline phosphate | - |
? | |
lysophosphatidylcholine + H2O | 8% of activity with sphingomyelin | Pseudomonas sp. | acylglycerol + choline phosphate | - |
? | |
N-dodecanoyl-4-nitrobenz-2-oxa-1,3-diazole-sphingomyelin + H2O | - |
Pseudomonas sp. | 4-nitrobenz-2-oxa-1,3-diazole-N-dodecanoylsphingosine + choline phosphate | - |
? | |
N-hexanoyl-4-nitrobenz-2-oxa-1,3-diazole-sphingomyelin + H2O | - |
Pseudomonas sp. | 4-nitrobenz-2-oxa-1,3-diazole-N-hexanoylsphingosine + choline phosphate | - |
? | |
sphingomyelin + H2O | - |
Pseudomonas sp. | N-acylsphingosine + choline phosphate | - |
? | |
sphingomyelin + H2O | SMase C causes hemolysis and thus is suspected to be a potential virulence factor | Pseudomonas sp. | N-acylsphingosine + choline phosphate | - |
? | |
sphingosylphosphorylcholine + H2O | 25% of activity with sphingomyelin | Pseudomonas sp. | sphingosine + choline phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 58110, deduced from nucleotide sequence | Pseudomonas sp. |
Synonyms | Comment | Organism |
---|---|---|
SMase C | - |
Pseudomonas sp. |
sphingomyelinase/hemolysin | - |
Pseudomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Pseudomonas sp. |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Pseudomonas sp. | deduced from nucleotide sequence | - |
6.33 |