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Literature summary for 3.1.4.12 extracted from

  • Miura, Y.; Gotoh, E.; Nara, F.; Nishijima, M.; Hanada, K.
    Hydrolysis of sphingosylphosphocholine by neutral sphingomyelinases (2004), FEBS Lett., 557, 288-292.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
phosphatidylserine 0.2 mM, approx. 108fold activation of sphingomyelin hydrolysis in the presence of 0.1% Triton X-100 Homo sapiens
Triton X-100 0.1%, approx. 108fold activation of sphingomyelin hydrolysis in the presence of 0.2 mM phosphatidylserine Homo sapiens
Triton X-100 0.1%, approx. 61fold activation of sphingomyelin hydrolysis in the presence of 0.2 mM phosphatidylserine Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
-
Bacillus cereus
expression of nSMase1 in HEK293 kidney cells Homo sapiens
expression of nSMase2 in HEK293 kidney cells Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
Homo sapiens
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sphingomyelin + H2O Homo sapiens
-
N-acylsphingosine + choline phosphate
-
?
sphingomyelin + H2O Bacillus cereus
-
N-acylsphingosine + choline phosphate
-
?

Organism

Organism UniProt Comment Textmining
Bacillus cereus
-
-
-
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant SMase Bacillus cereus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.46
-
sphingosylphosphocholine hydrolysis Bacillus cereus
0.49
-
sphingomyelin hydrolysis Bacillus cereus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
lyso-platelet-activating factor + H2O 103fold higher activity than with sphingomyelin in the absence of detergent Homo sapiens 1-alkylglycerol + choline phosphate
-
?
lyso-platelet-activating factor + H2O 55% of activity with sphingomyelin in the absence of detergent Homo sapiens alkylglycerol + choline phosphate
-
?
lysophosphatidylcholine + H2O 198fold higher activity than with sphingomyelin in the absence of detergent Homo sapiens acylglycerol + choline phosphate
-
?
lysophosphatidylcholine + H2O 580% of activity with sphingomyelin in the absence of detergent Homo sapiens acylglycerol + choline phosphate
-
?
sphingomyelin + H2O
-
Homo sapiens N-acylsphingosine + choline phosphate
-
?
sphingomyelin + H2O
-
Bacillus cereus N-acylsphingosine + choline phosphate
-
?
sphingomyelin + H2O very low activity in the absence of detergent Homo sapiens N-acylsphingosine + choline phosphate
-
?
sphingosylphosphocholine + H2O
-
Bacillus cereus sphingosine + choline phosphate
-
?
sphingosylphosphocholine + H2O 127fold higher activity than with sphingomyelin in the absence of detergent Homo sapiens sphingosine + choline phosphate
-
?
sphingosylphosphocholine + H2O 980% of activity with sphingomyelin in the absence of detergent Homo sapiens sphingosine + choline phosphate
-
?

Synonyms

Synonyms Comment Organism
neutral sphingomyelinase
-
Homo sapiens
neutral sphingomyelinase
-
Bacillus cereus
nSMase
-
Bacillus cereus
nSMase1
-
Homo sapiens
nSMase2
-
Homo sapiens